ورقة علمية منشورة : Bioproduction of silver-nano particles by Fusarium oxysporum and their antimicrobial activity against some plant pathogenic bacteria and fungi

Journal Article

AA, Al-Askar . 2013

• نوع عمل المنشور: بحث
• المجلة \ الصحيفة: Life Science Journal
• رقم العدد: 3
• رقم الإصدار السنوي: 10
• الصفحات: 6

مستخلص المنشور

Abstract: In the present study Fusarium oxysporium was used for bioproduction of nano-silver particles. Electron microscopey observation showed particles with sizes ranged from 16-30 nm. Antimicrobial activity of these particles was investigated against plant pathogenic bacteria (Erwina cartovra, E. amylovra, Dickya chransanthemi, D. dianthicale, Pectobacterium wasaibiae, P. atrosepticum chransanthem and P. wasabiae) and pathogenic fungi (Fusarium oxysporum, Alternaria alternata and Aspergillus flavius). Results revealed that the nanoparticles have high antibacterial activity against the examined bacteria and fungi when compared with the generic antibiotics. Protein separation was applied for the nano-silver particles suspension using SDS-PAGE and a protein with molecular weight about 26kDa was observed. When this protein was characterized using TLC in comparing with four different types of Aflatoxins (P1, G1, G2 and B2), results showed that the protein is unique and has a high molecular weight more than the four examined toxins. We can conclude that the nano-silver particles have selectivity for this active protein that improves the antimicrobial activity against the pathogenic fungi and bacteria.