ورقة علمية منشورة : Production, Purification and Optimization of Protease by Fusarium solani under Solid State Fermentation and Isolation of Protease Inhibitor

Journal Article

Al-Askar, A.A. . 2014

• نوع عمل المنشور: بحث
• المجلة \ الصحيفة: JOURNAL OF PURE AND APPLIED MICROBIOLOGY
• رقم العدد: 1
• رقم الإصدار السنوي: 8
• الصفحات: 239-250

مستخلص المنشور

Alkaline protease enzyme was produced under solid state fermentation by

Fusarium solani. Various agro-wastes were evaluated to check their potential utilization

in the solid state fermentation for protease production. In this connection, the results

showed that rice straw was the best agro-waste. The best enzyme activity was achieved at

7th day of incubation, pH 8, temperature 45 °C, inoculum’s size 200 μl/100 ml, agitation

speed at 140 rpm, and KNO3 as nitrogen source. The molecular weight of the obtained

purified enzyme is 30 KDa. A protease inhibitor protein was isolated and purified from

Rumex vesicarius L. The molecular weight of the inhibitor protein is 46 KDa and it

composes of 17 amino acids. The minimum inhibitory concentration was found at 20 μl

of the inhibitor per 40 μl of the enzyme. The obtained results showed that the inhibitor

can withstand the heat at 70°C for 30 min, and pH (5-9) for 72 h at 4°C without loss of

activity but it cannot be activated at low acidity less than pH 5 and very high temperature

more than 70°C. This study will serve to increase the understanding of factors that

control the production and inhibition of protease enzyme which may help in many

industrial aspects.