ورقة علمية منشورة : Streptomyces griseorubens E44G A Potent Antagonist Isolated from Soil in Saudi Arabia

Journal Article

Al-Askar, Abdulaziz A. . 2014

• نوع عمل المنشور: بحث
• المجلة \ الصحيفة: JOURNAL OF PURE & APPLIED MICROBIOLOGY
• رقم العدد: 2
• رقم الإصدار السنوي: 8
• الصفحات: 221-230

مستخلص المنشور

In this study, a wide survey was conducted along Saudi Arabia. One hundred

soil samples were collected from different 18 governorates representing different climatic

conditions. Five hundred and seventy strains of actinomycetes were isolated from the

collected soil samples. Among them, 225 were found to be antagonistic to the pathogenic

fungus (Fusarium oxysporum f. sp. lycopersici) with varying degrees. Only one isolate

designated E44G, had the strongest antagonistic activity against the tested fungus. The

taxonomic status of this isolate was established using Phenotypic and molecular methods.

The optimum nutritional and environmental conditions were studied to produce the

maximum yield of antifungal activity. The highest antifungal activity was obtained at 2nd

day of incubation, 7.5 pH, and 30 °C. Glucose was the best carbon source and yeast extract

was the best nitrogen source. In the present study, we report on a potential Streptomyces

strain which has antifungal and antibacterial activities and holds the potential for use

in studies of bioactive compounds as well as for possible use in biological control of

fungal and bacterial diseases of important crops.