Zoo-542 (Practical) Advanced Cytology

Practical contents
 
Zoo 542 Advanced Cytology  
 
1- A brief study of the concept of the cell
  Basic techniques in cell culture.  Biology of the cells in culture, origin and                                                             characterization, introduction about used cell lines (name, origin, source),                                            media for cell culture
2-            Cell growth and division
                Cell cycle analysis by FACS, concept and application.
                Cellular DNA Content Measurement by staining cells with propidium iodide and DAPI
3-           Cell viability assays e.g. MTT and NRU tests
4-            The structure of the chromosome
            Protocol for isolation of human metaphase chromosomes,
5-         1- Isolation of genomic DNA from cells.
            2-Estimation of quantity and quality of DNA by spectrophotometer and Agarose gel             electrophoresis
6. Isolation of RNA from cells.