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Dr Ali Daoud

Professor

Faculty

كلية العلوم
Department of Zoology College of Science King Saud University, Riyadh, Saudi Arabia
المنشورات
مقال فى مجلة
2013

Iron Oxide Nanoparticle-induced Oxidative Stress and Genotoxicity in Human Skin Epithelial and Lung Epithelial Cell Lines

Alarafi, Maqusood Ahamed, Hisham A. Alhadlaq, Javed Alam, M.A. Majeed Khan, Daoud Ali and Saud . 2013

Iron oxide (Fe3O4) nanoparticles (IONPs) have received much attention for their utility in biomedical applications such as
magnetic resonance imaging, drug delivery and hyperthermia. Recent studies reported that IONPs induced cytotoxicity in mammalian cells. However, little is known about the genotoxicity of IONPs following exposure to human cells. In this study, we investigated the cytotoxicity, oxidative stress and genotoxicity of IONPs in two human cell lines; skin epithelial A431 and lung epithelial A549. Prepared IONPs were polygonal in shape with a smooth surface and had an average diameter of 25 nm. IONPs (25-100 g/ml) induced dosedependent cytotoxicity in both types of cells, which was demonstrated by cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and lactate dehydrogenase leakage assays. IONPs were also found to induce oxidative stress in a dose-dependent manner, evident by depletion of glutathione and induction of reactive oxygen species (ROS) and lipid peroxidation. Comet assay revealed that level of DNA damage was higher with concentration of IONPs in both types of cells. Quantitative real-time PCR analysis showed that following the exposure of cells to IONPs, the expression levels of mRNA of caspase-3 and caspase-9 genes were higher. We also observed the higher activity of caspase-3 and caspase-9 enzymes in IONPs treated cells. Moreover, western blot analysis showed that protein expression level of cleaved caspase-3 was up-regulated by IONPs in both types of cells. Taken together, our data demonstrates that IONPs have potential to induce genotoxicity in A431 and A549 cells, which is likely to be mediated through ROS generation and oxidative stress. This study suggests that genotoxic effects of IONPs should be further investigated at in vivo level.

رقم المجلد
19
مجلة/صحيفة
Current Pharmaceutical Design - Bentham Science
الصفحات
6681-6690
مزيد من المنشورات
publications

Metal nanomaterials such as bismuth oxide nanoparticles (Bi2O3NPs) have been extensively used in cosmetics, dental materials,pulp capping, and biomedical imaging. There is little knowledge about…

بواسطة Ali Alamer1 & Daoud Ali2 & Saud Alarifi2 & Abdullah Alkahtane2 & Mohammed AL-Zharani3 & Mohamed M. Abdel-Daim2,4 & Gadah Albasher2 & Rafa Almeer2 & Nouf K. Al-Sultan2 & Abdulaziz Almalik1,5 & Ali H Alhasan1,5 & Saa
2021
publications

Silver nanoparticles (AgNPs) possess properties that are important for industrial and medical applications. This study is aimed to investigate intra-peritoneal toxicity of AgNPs at 26, 52 or 78 mg…

بواسطة Saud Alarifi, Daoud Ali, Mohammed A Al Gurabi & Saad Alkahtani
2017
publications

The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA…

بواسطة Saud Alarifi, Daoud Ali, Saad Alkahtani, and Rafa S. Almeer
2017