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Dr Ali Daoud

Professor

Faculty

كلية العلوم
Department of Zoology College of Science King Saud University, Riyadh, Saudi Arabia
المنشورات
مقال فى مجلة
2014

Iron Oxide Nanoparticles Induce Oxidative Stress, DNA Damage, and Caspase Activation in the Human Breast Cancer Cell Line

al, Saud Alarifi, Daoud Ali, Saad Alkahtani et . 2014

Broad applications of iron oxide nanoparticles require an improved understanding of their potential effects on human health. In the present study, we explored the underlyingmechanismthrough which iron oxide nanoparticles induce toxicity in human breast cancer cells (MCF-7). MTT (3-(4, 5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) and lactate dehydrogenase assays were used to examine mechanisms of cytotoxicity. Concentration- and time-dependent cytotoxicity was observed in MCF-7 cells. Iron oxide nanoparticles were found to induce oxidative stress evidenced by the
elevation of reactive oxygen species generation, lipid peroxidation, and depletion of superoxide dismutase, glutathione, and catalase activities in MCF-7 cells. Nuclear staining was performed using 4′, 6-diamidino-2-phenylindole (DAPI), and cells were analyzed with a fluorescence microscope. Iron oxide nanoparticles (60 μg/ml) induced substantial apoptosis that was identified by morphology, condensation, and fragmentation of the nuclei of the MCF-7 cells. It was also observed that the iron oxide NPs induced caspase-3 activity. DNA strand breakage was detected by comet assay, and it occurred in a concentration- and time-dependent manner. Thus, the data indicate that iron oxide nanoparticles induced cytotoxicity and genotoxicity in MCF-7 cells via oxidative stress. This study warrants more careful assessment of iron oxide nanoparticles before their industrial applications.

 

رقم المجلد
159
مجلة/صحيفة
Biological Trace Element Research - Springer
الصفحات
416–424
مزيد من المنشورات
publications

Metal nanomaterials such as bismuth oxide nanoparticles (Bi2O3NPs) have been extensively used in cosmetics, dental materials,pulp capping, and biomedical imaging. There is little knowledge about…

بواسطة Ali Alamer1 & Daoud Ali2 & Saud Alarifi2 & Abdullah Alkahtane2 & Mohammed AL-Zharani3 & Mohamed M. Abdel-Daim2,4 & Gadah Albasher2 & Rafa Almeer2 & Nouf K. Al-Sultan2 & Abdulaziz Almalik1,5 & Ali H Alhasan1,5 & Saa
2021
publications

Silver nanoparticles (AgNPs) possess properties that are important for industrial and medical applications. This study is aimed to investigate intra-peritoneal toxicity of AgNPs at 26, 52 or 78 mg…

بواسطة Saud Alarifi, Daoud Ali, Mohammed A Al Gurabi & Saad Alkahtani
2017
publications

The present work was designed to investigate the effect of palladium nanoparticles (PdNPs) on human skin malignant melanoma (A375) cells, for example, induction of apoptosis, cytotoxicity, and DNA…

بواسطة Saud Alarifi, Daoud Ali, Saad Alkahtani, and Rafa S. Almeer
2017