Analysis of pesticide residues in cucumber using highly sensitive ultra-performance liquid chromatography – tandem mass spectrometry

Journal Article

Mashhour, Ayman Abdul Ghfara, Saikh Mohammad Wabaidura, Zeid Abdullah Alothmana,*, Masoom Raza Siddiquia, Mohamed Habilaa, Majed Albokarib, Ibrahim . 2014

• رابط المنشور على الويب: https://www.academia.edu/8504307/01_malathion_2014
• الوسوم: Pesticide residue, Ultra Performance Liquid Chromatography, MS Detector, Malathion
• المجلة \ الصحيفة: woulfenia
• رقم العدد: 9
• رقم الإصدار السنوي: 21
• الصفحات: 119-138

مستخلص المنشور

In this study the determination of pesticide residues namely, malathion in cucumber collected from local markets in Riyadh city has been carried out. Samples of treated cucumbers were collected and analyzed during a 14 days period for
obtaining the residue amount of malathion. A liquid–liquid sampling technique has been applied to ultra-performance liquid chromatography/tandem mass spectrometry for analysis of residual malathion in cucumber. Multiple reactions monitoring for two transitions was acquired while the transition with higher sensitivity used for quantification and the transition with lower sensitivity used for confirmation analysis of malathion. The proposed method has been validated by establishing performance  parameters such as percent recovery, precision, linear ranges, limits of detection (0.0042 μg/ml) and limits of quantification (0.014 μg/ml). The obtained recoveries of malathion in fresh cucumber samples of 10 replicates were found to be in the range
between 96.9 and 101.2% (%RSD ˂ 5.2) for two fortification levels (0.02 and 0.1
mg/kg). The intra-day and inter-day RSD values were obtained between 1.7 and
2.1%. The extracted cucumber samples have shown different level of contamination
upto certain time interval (7 days) and after that no residues between 11-14 days
were detected.