علي راشد الحوشاني

المؤهلات الدراسيه

• دكتوراه جامعه كاليفورنيا، ارفاين (٢٠٠٩-٢٠١٤)
• بكالوريس جامعه الملك سعود  . الرياض ( ١٩٩٩-٢٠٠٥)
• شهاده في الابحاث الاكلينيكيه، جامعه كاليفورنيا، ارفاين(٢٠١٢-٢٠١٣)

آللاهتمامات البحثيه

• الخلايا الجذعيه ، خلايا الدم الجذعيه ودورها في سرطان الدم
• تطوير وتحليل تسلسل الجيل الجديد من الاحماض الامينيه  
• دراسه هيكله البروتينات الغشاىيه 
• تعيين تركيب البروتين الغشائي والدراسات المميزة له عن طريق الرنين المغناطيسي وأشعة إكس والدائرة المزدوجة اللون (CD)
• تصنيع البروتينات ياستخذام نظام خلوي
• استخدام الخوادم لاستكشاف مستقبلات للادويه وتحديد آليه عمل الدواء ياستخدام High Ambiguity Driven biomolecular DOCKing ((HADDOCK

النشر العلمي

• Glutamate provides a key structural contact between reticulon-4 (Nogo-66) and phosphocholine. Biochim Biophys Acta. 2014 May 24.
• Revealing p53 Rescue Mutant Mechanism (in progress).e
• The Nogo Model: Nogo Receptor Complex (in progress).e​

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Education and Degrees

• University of California Irvine, Irvine, CA                                                                              (Sep2009-2014)
• Ph.D. in Pharmacological Science, Pharmaceutical Science Dept.
• Clinical Research Certificate (2013) School of Medicine-UCI Extension Joint Program

King Saud University, College of Pharmacy, Riyadh, Saudi Arabia                                        (May1999-July2005)

• B.S. Pharmacy (2005)

Research and Laboratory Practice 

• Stem Cells techniques. Including sterile hES tissue culture, preparing mouse embryonic fibroblast (MEF) feeder plates, Matrigel (MEF-free) culture plates, multiple enzymatic and mechanical hES cell passaging techniques, embryoid body formation, nucleofection by green fluorescent protein (GFP), fluorescent microscopy with immunostaining (SSEA4 & OCT4) as well as alkaline phosphatase staining, and karyotyping hES with fluorescent microscopy using DAPI nuclear stain , Sue & Bill Gross Stem Cell Research Center
• Aptamers Selection for target protein by in vitro Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
• Analyze and manipulate Next Generation Deep sequence data by Cluster Database at High Identity with Tolerance (CID-HIT) and Amin Sorting Data, in-house developed software (ASD).
• Performed recombinant protein expression and purification, including membrane proteins.
• Elucidated mechanism of Protein-Receptor interaction by High Ambiguity Driven biomolecular DOCKing (HADDOCK).
• Determined binding site and binding affinity for small molecule-Protein, Nucleic Acid-Protein and Protein-Protein interactions by Mass Spectrometry (MOLDI-TOF), Nuclear Magnetic Resonance (NMR), and Electrophoresis Mobility Shift Assay (EMSA).
• Studied protein folding and secondary structure by Circular Dichroism (CD).
• Used NMR to elucidate the mechanism of how some of the rescue mutants restore the function of wild type p53.
• Worked to solve the structure of Jaagsiekte sheep retrovirus (JSRV) cytoplasmic tail to develop better understanding of its ability to transform normal lung cells to cancer cells.