BCH 312 Biochemical Calculations

To provide a basic understanding on how to calculate problems in biochemistry. Classification of elements and compounds and definition of solution and types of solutions. Properties of water and ionization and amphipathic of water and types of solution concentration. Saturated solution, unsaturated solution, supersaturated solution, stock solution, concentrated solution, diluted solution and standard solution. How to prepare solution, expressions of concentration of solutions, qualitative and quantitative expressions of concentration. Molarity, normality, weight per volume percent, parts per million (ppm), parts per billion (ppb), osmality, ionic strength. Neutralization and titration relations. Preparing laboratory solutions and reagents, How to make standard, diluted, concentrated solution. Definition of the biochemical buffers, laboratory buffers, factors affecting the buffers, buffer capacity, criteria for good buffer solution. Adjusting the pH of a buffer, bringing a solution to the proper pH, using buffers, assuring the quality of a solution. Definition and derivation of Henderson-Hasselbalch equation, Henderson-Hasselbalch equation limitations. UV-visible spectroscopy, introduction of spectrometric analyses, transmission characteristics of cell materials, types of spectrophotometric cells (Cuvettes). Definition and derivation of Beer Lambert law, spectrophotometric analytical method, how to make standard curve, absorbance and transmittance, precision and accuracy, protein concentration determinations. Statistical analysis of data, quantitative and qualitative research methods, sampling and sample design, error sources of experimental measurement.