CLS 231

Department                  :    Clinical Laboratory Sciences

 Course number            :     CLS 231

 Course title                  :     Clinical Analytical Chemistry   

 

 

 Course description : 

             The course involves a review of fundamental concepts of volumetric anaylysis as applied to biological fluids. The theoretical and practical aspects of different types of titrimetric analysis including acid-base, complexometric and precipitation titrations are studied .

 

 

CLS   231                              Laboratory Schedule     

 

Week                                        subject

 

1 .                   Basic laboratory equipment and procedures safety rules   

 2 .                  Standardisaion of sodium hydroxide and hydrochloric acid  

 3 .                   Determination of bicarbonate in blood   

 4 .                   Mercurimetric determination of blood and urine chloride 

 5 .                   Determination of blood glucose by a Redox titration method   

 6 .                   Complexometric determination of calcium in milk  

 7 .                  Determination of the ionisationconstant of a weak acid 

 8 .                   Determination of chloride by the vohlard method

 9,10                Gravimetric determination of chloride

 11 .                 Basic principles of visible spectrophotometry 

 12 .                Revision  

 13 .                 FINAL LABORATORY EXAMINATION 

 

 

 

Lab Work:

Semester :15

Lab Final Exam:15

 

 

References:

1. David L. Nelson and Michael M. Cox, Lehninger Principles of Biochemistry, W. H. Freeman; 4th edition (April 23, 2004).

2. Pamela C. Champe and Richard A. Harvey, Lippincott’s illustrated reviews: Biochemistry, Lippincott Williams & Wilkins; 2 Sub edition, 1994.