CLS 411 Bacteriology 1

Practical Course Specifications

  

Basic Information

-Title of the programs: CLS

-Title of the course: Clinical Bacteriology  1

-Code number of the course:  CLS 411

-Credit hours:  1 hour

-Contact hours:  3 hours

-Academic year: …1432-1433……2^st semester

-Level:  seven

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Overall aim of the course:

1-   

 Learn to identify the given organisms based on their morphology, growth properties and biochemical reaction.

2-   

Learn how to perform different identification test and understand their principle.

3-   

 Learn about different media used for culturing and their role in identification.

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Intended learning outcomes of the course: (Skills)

·     

Practical skills:

1-   

Perform safe laboratory practice.

2-   

Learn the aseptic technique and how to apply it to a variety of laboratory techniques.

3-   

Learn and perform gram stain as many as possible, to be able to master the technique.

4-   

Learn the proper way to focus a microscope and clean it afterword.

5-   

Learn and practice the proper streaking of culture plate, and how to obtain isolated colonies.

Interpersonal skills:

1-   

Learning team work by sharing with other student the reagent and things used in the lab.

2-   

Learn how to take responsibility on organizing and cleaning their area after they finish work.

 

 

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Course contents (Time schedule)

Week	Practical lesson	No. of hours
1	Antibiotic Sensitivity (Sold Method)	2-3
2	Antibiotic Sensitivity (Broth Dilution Method)	2-3
3	Staphylococci	2-3
4	Staphylococci (Continue)	2-3
5	Streptococci	2-3
6	Streptococci (Continue)	2-3
7	Niesseria	2-3
8	Listeria	2-3
9	Corynebacterium	2-3
10	Bacillus	2-3
11	Mycobaterium	2-3
12	Revision	2-3
13	Final Exam	2

 

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Teaching Methods:

1-   

Power point presentation, with pictures of what the student should see in the practical session.

2-   

Demonstration of gram stain, different culture plates and different test when available.

3-   

Student should perform gram stain, subculture almost every lab to master the technique.

4-   

Student should perform different test when possible.

 

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Assessment methods:

1-   

Quizzes 10 marks

+ 30 marks for final = total 40 marks for practical.

2-   

 Checking gram stain for student and point out the different mistakes they make.

3-   

Assessment of the student work in every lab (streaking, focusing of microscope, other test) and coaching.

4-   

Final spot practical exam.

 

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References:

1-District Laboratory Practice in Tropical Countries Part 2-2^nd Edition.

2-Recommended websites:

·     

http://pathmicro.med.sc.edu/book/bact-sta.htm.

·     

www.asm.org.   (American Society for Microbiology).