CLS 417

Clinical Practice of  Microbiology 

King Khalid University Hospital - Department of Microbiology

Wed 10:00 - 12:00

COURSE DESCRIPTION 
Student training in routine microbiology laboratory in a university hospital . Training includes safety precautions in laboratory , media preparation , sterilization , specimens collection methods , processing of specimens for culture , isolation and identification of organisms by conventional and rapid methods , clinic bacteriological correlation and antibiotic sensitivity . The last part of the course includes a tour for rapid preview in clinical immunology mycology and virology laboratories in university hospital of the routine diagnostic methods and the most recent or most rapid ones

WEEKS                       SUBJECTS 
  1
                                    media  -basic constituents -preparation -sterilisation -types       
2,3                              Urine  -specimen collection -processing for chemical analysis -pathogens isolated  -processing        

                                              routine culture    -sterile pyuria 
          
    4                         Blood   -indications for blood culture -time for taking blood culture -technique of blood collection for 
                               culture - blood culture media -organisms isolated from blood  -processing for culture

 5-6        
              Sputum -indications for sputum culture
-common pathogens -collection methods -processing for culture in routine respiratory tract infections -processing of culture in pulmonary tuberculosis by conventional methods and BACTEC -antimycobacterial susceptibility testing by conventional methods an using BACTEC 
7-8-9   
                 Stools -indications for culture -pathogens isolated -processing of stools for culture -isolation and identification of different oirganisms -preparation for parasitological examination   
10-11     
               General bench specimens : CSF PUS EAR CONJUNCTIVA GENITAL NASAL TROAT -indications for taking these specimens -collection methods -common pathogens -processing for culture 
12  
                      commonly used serological tests in clinical bacteriology -widal test -C.F.T. -ASO   
-serological test of syphilis specific and non specific                                                                                                                 13     
             Rapid review in diagnosis in common viral infections  -tissue culture types  -CPE  -EIA

.Choose the most appropriate statement for each of the following: 

1.      *The specimen for the detection of Streptococcus pyogenes-pharyngitis Is::
    a. throat swab.          b saliva          c. buccal cavity swab   
    d. Sputum                                e.  blood culture.  
 
2.      Common contaminants of blood cultures:
 a. Gram-negative rods                b. Coagulase-negative staphylococci
 c. Staphylococcus aureus          d. Anaerobes     e. None of the above
 
3.      Which organism is Gram-variable (resists discoloration):
 a. Staphylococcus                   b.  Escherichia          c. Streptococcus  
 d. Gardnerella                             e. Enterococcus
 
4.      The time needed for growth appearance on agar plate of most  bacteria is:
   A.48 hours.     B. 24 hours.     C. 72  hours.  D. one week.     E. two weeks.
 
5.      Rapid Candida albicans-identification in a cervical- exudates is by :
a. latex agglutination test          b. special culture
c. Gram stain and 'wet prep' with 10% KOH         d. none of the above
 
6.      The best specimen to isolate CMV in pregnant women is:
         a. Serum     b. Throat swabs      c. CSF             d. Urine
 
7.      Which tests indicates the presence of an infectious virus in a sample:
 a. Hemagglutination Inhibition (HAI)
 b. Immunofluoresence or Fluorescence assays (IF or FA )
 c.Neutralization Tests (NT)    d. Complement Fixation Test (CF)      e. ELISA
 
8.      *Which of the following statements is (NOT TRUE):?
  a.Mycobacterium tuberculosis is acid-fast. 
   B.Streptococcus pneumoniae is soluble in bile 
   c.Staphylococcus epidermidis is coagulase negative.
   d. Escherichia coli grow as LF on MacConkey agar.  
   E. Shigella species are motile in SIM medium.
 
9.      Encapsulated microorganisms rapid diagnosis is by:
              a.Rapid agglutination test        b.Kinyoun stain (cold Z.N.stain)   
   c.India Ink stain     d.Bile-esculin and 6.5% salt medium    
   e.Carbohydrate fermentation
 
10.  *Enterobacteriaceae diagnosis is by :
    a.SSS Co-agglutination test        b.Kinyoun stain (cold Z.N.stain)   
    c.India Ink stain         d.Bile-esculin and 6.5% salt medium     
   e.Carbohydrate fermentation
 
11.  *Differentiation of Group D enterococci from Group D non-enterococci is by:
 a.Inhibition of growth by bacitracin        b.Fermentation of lactose 
 c.Production of CAMP factor                 d.Growth in 6.5% salt broth 
 
12.  *Streptococcus agalactiae(GBS) differ from other streptococci by:
  a.Inhibition of growth by bacitracin        b.Fermentation of lactose 
  c.Production of CAMP factor                 d.Growth in 6.5% salt broth  
 
13.  *A positive CRP test means the patient has:
 a. bacterial infection   b. viral infection 
 c. fungal infection     d. Any of above is true
 
14.  *The causative organism of Rheumatic fever is:
               a. Staphylococcus aureus              b.Streptococcus agalactaiae    
               c.Streptococcus pyogenes             d.Neisseria gonorrheae
 
15.  *The BCG vaccine to prevent tuberculosis is:
  a. whole killed cells of M. bovis      b. live attenuated cells of M .bovis  
  c. a toxoid                                       d. none of the above
 
Answer:1(a),2(b),3(d),4(b),5(c),6(d),7(c),8(E),9(c),10(e),11(d),12(c),13(d),14(c)

refernce book
medical microbiology
24th edition
jawetz,melinckand adelbergs