The Lab's Goals
The Course’s Goals:
(1) Train the students on different experimental procedures in metabolism .
(2) To learn students some valuable skills in the field of biochemistry.
(3) Gain ability to read and explanation the result .
The distribute of experiments during semester
|
Week No. |
Title of experiment |
|
1 |
Introduction ,General Laboratory and Safety Guidelines |
|
2 |
ISOLATION OF GLYCOGEN FROM LIVER |
|
3 |
ENZYMATIC HYDROLYSIS OF GLYCOGEN AND DETERMINATION OF GLUCOSE |
|
4 |
ESTIMATION OF ARGINASE ACTIVITY IN LIVER EXTRACT |
|
5 |
TRIGLYCERIDE DETERMINATION |
|
6 |
HDL-CHOLESTEROL and totlal Chlo. DETERMINATION |
|
7 |
LIPASE ASSAY(USING TURBIDIMETRIC METHOD) |
|
8 |
Estimation of Glutathione in Plasma |
|
9 |
Orall Glucouse tolerance test |
|
10 |
Galactocemia |
|
11 |
Phenylalanemia |
|
12 13 |
Ammonia estimation in plasma D-Xylose absorbance test |
*The total marks are 100
40 mark for final ( 15 theoretically & 25 practical)
25 mark for mid term
15 mark for report
15 mark for Quiz
5 mark for practical work in the lab.
Reference:
-The Biochemistry Department (2008). Laboratory Manual in General Biochemistry : University of Santo Tomas.
-Johnson, C.R., Miller, M.J., Pasto, D.J. (1998). Experiments and Techniques in Biochemistry. United States of America: Prentice Hall Inc.
-Landgrebe, J.A. (1993). Theory and Practice in the Biochemistry Laboratory: with Microscale and Standard Scale Experiments. California: Wadsworth Inc.
- http://www.biology.arizona.edu