مكان وزمان المحاضرة ليوم الثلاثاء
طلابي الأعزاء,
بناء على رغبة جميع الطلاب الحاضرين في محاضرة يوم الثلاثاء (8-8:50) تم تغيير وقت المحاضرة الى يوم الاثنين من الساعة 11 الى الساعة 11:50
وفقكم الله,,
طلابي الأعزاء,
بناء على رغبة جميع الطلاب الحاضرين في محاضرة يوم الثلاثاء (8-8:50) تم تغيير وقت المحاضرة الى يوم الاثنين من الساعة 11 الى الساعة 11:50
وفقكم الله,,
Acidic fibroblast growth factor (FGF-1) is an angiogenic protein which requires binding to a polyanion such as heparin for its mitogenic activity and physicochemical stability. To evaluate the extent to which this heparin dependence on solution stability could be reduced or eliminated, the structural integrity and conformational stability of ten selected FGF-1 mutants were examined as a function of solution pH and temperature by a series of spectroscopic methods including circular dichroism, intrinsic and extrinsic fluorescence spectroscopy and static light scattering.
The structural integrity and conformational stability of an IgG1 monoclonal antibody (mAb), after partial or complete enzymatic removal of the N-linked Fc glycan, were compared with the untreated mAb over a wide range of temperature (10°C–90°C) and solution pH (3–8) using circular dichroism, fluorescence spectroscopy, and static light scattering combined with data visualization employing empirical phase diagrams. Subtle-to-larger stability differences between the different glycoforms were observed.
The student basic biochemistry and pharmacy education will be expanded with current concepts in biochemistry, molecular biology, analytical techniques, drug development, delivery and formulation relevant to the use and development of biotechnology-derived products, including protein and nucleic-based pharmaceuticals. The course produced pharmaceutical, with special emphasis on their clinical use and application, including advantages and current limitation. Their application in both drug and vaccine therapy will be covered using case studies.