Bioimaging of transcriptional activity of microRNA124a during neurogenesis

Journal Article

Kim, Hae Young Ko, Jonghwan Lee, Yong Seung Lee, Youngsok Choi, Ali, B.A., Abdulaziz A Al-Khedhairy, Soonhag . 2015

• Publication Work Type: Research Article
• Magazine \ Newspaper: Biotechnology letters
• Volume Number: 37
• Pages: 2333–2340

Publication Abstract

Objectives A special vector system was developed to

monitor the in vitro and in vivo endogenous level of a

primary transcript of miR124a during neuronal

differentiation

Results The upstream regions of miR124a were

fused with luciferase (Gluc) and their activity was

measured. During neurogenesis of P19 cells, the

primary transcript level of miR124a was increased

1.5-times compared to the undifferentiated P19 cells.

P19 cells grafted to nude mice exhibited the same

pattern of luciferase activity in vivo as they did

in vitro.

Conclusion The expression of primary miR124a

during neurogenesis was successfully imaged by

in vitro and in vivo luciferase reporter gene-based

method.

Keywords Bioluminescence imaging In vivo

imaging MicroRNA124a Neurogenesis Promoter