Dual optical biosensors for imaging microRNA-1 during myogenesis

Journal Article

SH, Kang, WJ; Cho, YL; Chae, IR; Lee, JD; Ali, BA,; AlKhedhairy, AA; Lee, CH; Kim, . 2012

• Publication Work Type: Research article
• Magazine \ Newspaper: Biomaterials
• Volume Number: 33
• Pages: 6456-6467

Publication Abstract

Dual optical microRNA (miRNA) imaging systems, bioluminescent reporter gene (a signal-off mechanism)-

or fluorescent molecular beacon (MB) (a signal-on mechanism)-based miRNA imaging system, have individually

allowed us to sense miRNA biogenesis in a noninvasive and iterative manner. Both of these imaging

systems have shortcomings with respect to image quality. Therefore, we designed a dual optical imaging

systems by simultaneous imaging of a miRNA-1 reporter gene (CMV/Gluc/3xPT_miR-1) and miRNA-1MB in

a single cell to overcome these limitations and used it to visualize miRNA-1, a highly expressed miRNA in

cardiac and skeletal muscle. During myogenic differentiation of C2C12 cells, the bioluminescence intensity

from CMV/Gluc/3xPT_miR-1 revealed a miRNA-1-dependent gradual decrease and the fluorescence

intensity frommiR-1MBdemonstrated a miRNA-1-dependent gradual increase both in vitro and in vivo. The

dual miRNA-1 imaging systems, which provides the complementary imaging information about miRNA

biogenesis, could be useful to sense miRNA expression during various biologic processes