Dina M. Metwally * , Mashael A. Al-Damigh , Isra M. Al-Turaiki and Manal F. El-Khadragy (2019).Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia

Journal Article
, Dina M. Metwally 1,2,* , Mashael A. Al-Damigh 3, Isra M. Al-Turaiki 4 and Manal F. El-Khadragy 1,5 . 2019
Magazine \ Newspaper: 
Animals
Pages: 
9
Publication Abstract: 

 Sarcocystosis is induced by species of Sarcocystis, which is an intracellular protozoan parasiteinthephylumApicomplexa. ThediversityandimportanceofSarcocystisspeciesinsheepand goats in Saudi Arabia are poorly understood. In this study, the tongue, esophagus, heart, diaphragm, and skeletal muscles were collected from 230 sheep and 84 goats, and the tissues were examined for the presence of Sarcocystis species by macroscopic examination and light microscopy. Microscopic Sarcocystisspeciescystswerefoundinbothsheepandgoats. Transmissionelectronmicroscopy(TEM) revealed S. tenella in sheep and S. capracanis in goats. Sarcocystis species were confirmed for the first time in Saudi Arabian sheep and goats by molecular testing. S. capracanis was most closely related to S. tenella, with the COX1 sequences sharing 91.7% identity. A phylogenetic analysis produced similar results and indicated that the Sarcocystis isolates were within a group of Sarcocystis species in which dogs were the final host. Finally, the Sarcocystis species cysts from sheep and goats could be grouped together, indicating that they were strongly related.