تجاوز إلى المحتوى الرئيسي
User Image

Dr. rer. nat Elsayed Ahmed Elsayed

Associate Professor

Faculty Staff Member

كلية العلوم
Building 5, AB107
المنشورات
مقال فى مجلة
2014

Aeration Rate Effect on the Growth Kinetics, Phytase Production and Plasmid Stability of Recombinant Escherichia coli BL21 (DE3)

, Nor Zalina Othman, Elsayed EA, Roslinda Abd Malek, Solleh Ramli, Helmy J. Masri, Mohamed R. Sarmidi1, Ramlan Aziz, Mohamad A. Wadaan, Rajni Hatti-Kaul and Hesham A. El Enshasy . 2014

Phytase production using Escherichia coliBL21 (DE3), a recombinant strain harboring a plasmid encoding thermostable Bacillusphytase, in semi-industrial scale was studied in this work. Among the factors needed to be considered in order to achieve high enzyme yield, aeration rate plays an important role. Suitable aeration is required to supply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv-1 min-1), on the kinetics of cell growth and phytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tank bioreactor was investigated. The highest cell concentration of 3.81 gL-1 concomitant with the maximal total phytase production of 15.63 UmL-1 were obtained in a bioreactor of aeration rate 3.0vv-1 min-1. At this high aeration rate, a maximal specific growth rate (µ max) and the maximal specific phytase production of 0.33 h-1 and 4102Ug-1, respectively, were achieved

رقم المجلد
8
رقم الانشاء
4
مجلة/صحيفة
J. Pure & Appl. Microbiol
الصفحات
2721-2728
مزيد من المنشورات
publications

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…

بواسطة El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy M
2018
publications

A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
agents. Nitrosation of methyl 5-amino-2-hydroxybenzoate or 1-…

بواسطة El-Naggar M, El-Shorbagi A-N, El-Naggar D, Amr AE, Al-Omar M, Elsayed EA
2018
publications

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…

بواسطة Elsayed EA, El Enshasy HA
2018