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Dr. rer. nat Elsayed Ahmed Elsayed

Associate Professor

Faculty Staff Member

كلية العلوم
Building 5, AB107
المنشورات
مقال فى مجلة
2015

Enhanced production of Rifamycins B and SV by medium supplementation with Vermiculite

Enshasy, Elsayed A. Elsayed, Hoda Omar and Hesham A. El . 2015

Rifamycins are  broad spectrum and important antibiotics used in the treatment of  pathogenic diseases. They are generally  produced  by  the  fermentative  cultivation  of  Amycolatopsis  mediterranei,  using  submerged  cultivation 
system. The medium composition greatly influences the antibiotic production. Trace elements are one ofthe major factors  need  to  be  optimized  to  enhance  the  production  of  rifamycins.  In  the  present  work,  we  investigated  the possible  improvement  of  rifamycins  B  and  SV  production  through  the  addition  of  vermiculite  (dehydrated magnesium-aluminum-ion silicate) as a cheap and affordable source of trace elements. The obtained results showed that the addition of 20 g/L vermiculite gave the highest volumetric production of rifamycins B and SV  (767.5 and 
1265  mg/L for  Rif  B  and SV,  respectively).  Moreover, addition of  vermiculite  at the beginning  of the  cultivation resulted  in  the  highest  volumetric  productivities  in  comparison  to  control  cultivation  (770  and  1305  mg/mL  of rifamycin  B  and SV, respectively). Comparative growth curve kinetics between vermiculite-supplemented  medium and  vermiculite  free  medium  demonstrated  that  vermiculite  significantly  enhanced  cell  growth,  cell  growth  rate, glucose  consumption  rate,  as  well  as  the  production rates  of  both  rifamycins  B  and  SV  compared  to  cultivation without vermiculite. In addition, vermiculite supplementation increased both maximal volumetric productivity and production rates increased by about 32 and 52% for rifamycin B and SV, respectively, as compared to vermiculitefree cultivation.

رقم المجلد
7
رقم الانشاء
4
مجلة/صحيفة
J. Chem. Pharm. Res
الصفحات
1089-1094
مزيد من المنشورات
publications

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4…

بواسطة El Enshasy, Elsayed EA, Suhaimi N, MalekRA, Esawy M
2018
publications

A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
agents. Nitrosation of methyl 5-amino-2-hydroxybenzoate or 1-…

بواسطة El-Naggar M, El-Shorbagi A-N, El-Naggar D, Amr AE, Al-Omar M, Elsayed EA
2018
publications

Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…

بواسطة Elsayed EA, El Enshasy HA
2018