In vitro assessment of anticancer properties of Moringa peregrina essential seed oil on different cell lines
Journal Article
M, Elsayed EA, Sharaf-Eldin MA, El-Enshasy HA and Wadaan . 2016
Magazine \ Newspaper:
Paksitan J. Zool
Issue Number:
3
Volume Number:
48
Pages:
853-859
Publication Abstract:
Moringa peregrina belongs to the Moringaceae family and is native to the western part of Saudi Arabia and the eastern part of Egypt, from where it has been transferred to tropical and subtropical countries. The Moringa trees are highly appreciated for their nutritional and medicinal properties. In the past two decades, research has focused on investigating different parts of Moringa as a source for anticancer preparations. However, M. peregrina extracts were only evaluated for their antioxidant and anti-inflammatory activities, and the literature contains few (or no) information about the cytotoxic properties reported for M. peregrina seed oil. Accordingly, the current work aimed to evaluate the potential anti-proliferative properties of essential oil obtained from M. peregrina and its effects on various cell types. Different cell lines were exposed to increasing concentrations from the seed oil (0.15 to 1000 μg/mL) for 24h, and cell toxicity was evaluated. Results revealed that all cell lines under investigation were significantly affected depending on the used oil concentration. Furthermore, cell lines showed different response to the treatment with oil according to cell type. Additionally, HepG2 and HeLa cells showed the highest response to the applied essential oil, followed by MCF-7, CACO-2 and L929, where the toxicity percentages at the maximal oil doses recorded 75.82, 75.35, 67.89, 57.50 and 53.43%, respectively. The IC50 value obtained for HeLa cells was 366.3 μg/mL, and increased by 1.7- and 2.0-fold for HepG2 and CACO-2 cells, respectively. The present results suggest that essential oil isolated from M. peregrina seeds has potential cytotoxic properties against different cancer cell lines