Optimization of fed-batch cultivation strategy for extracellular -amylase production by Bacillus amyloliquefaciens in submerged culture
El-Enshasy, E A Elsayed, H G Omar,S A Galil and H A . 2016
Alpha amylase (E.C. 3.2.1.1) is one of the most important starch-degrading enzymes and widely applied in pharmaceutical, food, feed, detergent, textile and bio refinery industries. The present work was focused on optimization of -amylase production using the bacteria strain Bacillus amyloliquefaciens in submerged cultivation system. The results
showed that, the optimal soluble starch concentration for maximal enzyme production of 1950 kat.L-1 was 20 g.L-1. Compared to shake flask, bioreactor batch cultivation yielded higher volumetric enzyme production of 4125 kat.L-1 (about 1.84-folds higher than the value obtained in shake flask). In addition, fed-batch cultivation with two feeding strategies (constant feeding and increased feeding) was also designed to improve the production process. The maximal volumetric enzyme production of 8160 kat.L-1 was obtained in increased feeding strategy after 36 hours of cultivation. This value was almost 98 and 18% higher than the enzyme produced in batch and constant feeding fed-batch cultivations, respectively
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A series of azocompounds containing methyl salicylate 4a–k and 1-naphthyl moiety 6–8 was synthesized and tested as anticancer
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Invertase (β-D-fructofuranoside fructohydrolase; EC 3.2.1.26) constitutes an improtant microbial enzyme with wide applications in differnt food and pharmaceutical sectors. The present work used…