Isolation, Identification and Medium Optimization for Tyrosinase Production by a Newly Isolated Bacillus subtilis NA2 Strain

Journal Article
Danial, Elsayed Ahmed Elsayed, Enas Nabil . 2018
Magazine \ Newspaper: 
J Appl Phar Sci
Issue Number: 
9
Volume Number: 
8
Pages: 
93-101
Publication Abstract: 

Tyrosinase (EC 1.14.18.1, monophenol, O-diphenol: oxygen oxidoreductase) is a copper-metallo-bifunctional enzyme,
which catalyzes the O-hydroxylation and oxidation of monophenols to quinones. Tyrosinase plays an important role
in the immune defense mechanisms of many prokaryotic and eukaryotic organisms. It also finds many applications in
antioxidants and therapeutic fields. In the present work, a newly isolated tyrosinase-producing strain (Bacillus subtilis
NA2) was identified using 16S rDNA and BLAST analysis. The primary cultivation medium produced tyrosinase at
416.67 U/min/mL with a yield coefficient (YP/X) of 111.75 × 103 U/g cells. Furthermore, the cultivation medium for
the production of tyrosinase was optimized. The final optimized cultivation medium composed of (g/L): sucrose, 10;
KNO3, 10; K2HPO4, 0.5; MgSO4.7H2O, 0.25; tyrosine, 10 and the initial optimized pH of the cultivation medium was
8.5. It has been shown, that the final optimized medium with the optimized pH resulted in an increase of about 39%
in tyrosinase production from the initial un-optimized conditions. The new tyrosinase producing strain was identified
and its volumetric production was significantly increased after the successful optimization of the production medium