Evaluation of Cytotoxic and Anti-Tumor Activities of Coscinium fenestratum Extract
Sandeep, Divyasree . 2014
Objective: To evaluate the anti tumor activity of Coscinium
fenestratum extract under in vitro and in vivo conditions. Materials
and Methods: The cytotoxic activity of C. fenestratum extract was
tested on HeLa cells by MTT assay. The apoptosis-inducing activity
of the extract was measured via mitochondtial membrane potential
assay, acridine orange and ethidium bromide dual staining method,
DNA fragmentation assay and caspase assay. The in vivo antitumor
activity of the extract was tested against developed solid tumor in
mammalian system. Results: The extract showed significant antiproliferative
activity and is dose dependent. The extract treatment
clearly showed an increasing number of apoptotic events both in
HeLa cells and DLA cells and collapsed the mitochondrial membrane
potential and these effects were higher than that of the positive
control taxol. The cellular DNA was cleaved in to multiple
fragments. Membrane blebbing and nuclear fragmentation was also
observed after the extract treatment. C. fenestratum also triggered the
release of mitochondrial cytochrome -c and activation of caspases 3
and 9 in HeLa cells, indicative of its apoptotic potential and was
found to be highly effective in tumor regression. Conclusions: These
finding suggested that the potential antitumor activity of C.
fenestratum extract.
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