Course Description

Department            :         Clinical Laboratory Sciences

Course Number     :         CLS 422

Course Title           :         Cytopathology 

Credit Hours          :         1 + 1 = 2

 

 

Course Description:

This course will provide the student with the basic knowledge of the theory and practical aspect in the diagnosis of tumour cells and non-malignant conditions. This can be achieved by microscopic examination of smears prepared from exfoliated cells or fine needle aspirated material. The course will enable the student to identify through the microscope, the normal and malignant cells by which malignant tumours can be diagnosed. The students also will be trained in the different technical methods applied in smears preparation and self precautives from contacting infections.

 

 

CLS 422:   Lectures Outline

 

Weeks                                        Subjects 

1,2.                            Introduction to cytology

                    The anatomy & histology of the female genital tract

 

3.                               The normal constituents of Pap-smear

                                  The normal cytology of female genital tract and other

                                   cells of the body

 

3.                               Hormonal Cytology

 

4,5,6.                         Inflammation: Criteria and causes

                                  Diagnostic cytology of different inflammatory

                                  Smears

 

7.                               Premalignant and Malignant criteria

 

8.                               Diagnosis of malignancy in Pap smear  “cytology of

                                  malignant cells”

 

9.                                 Introduction to non-gynaecology cytology and the diagnosis

                                    of neoplastic and non- neoplastic cells from many parts of

                                    the body

 

10.                               Sputum, bronchial wash and bronchial brush cytology

 

11.                               Body fluids cytology “cytology of serous effusion”

 

12.                               Fine needle aspiration cytology (general information)

 

13.                             Urine, oral and C.S.F cytology

 

 

 

CLS 422:   Laboratory Schedule

 

Weeks                                        Subjects

 

1.                                 Introduction to cytology laboratory work

 

2.                                 Screening histology and cytology slide of the normal

                                    cellular constituents of Pap smear

 

3.                                 Papanicoloau staining method and screening cytology

                                    Slides for normal cytology

 

4.                                 Practice screening of hormonal cytology

 

5.                                 Screening abnormal (inflammatory) cervical smears

                                    and identifying the causes of inflammation

 

6,7.                              Screening smears and identifying benign and

                                     malignant cells

 

8.                                 Smear preparation from sputum and non-

                                    gynaecological samples

 

9.                                 Screening smears for the identification of malignant

                                    cells in sputum, bronchial wash and bronchial brush

                                    smear

 

10.                               Screening body fluid smears for malignant cell

                                    Identification

 

11.                               Urine cytology, Oral cytology, C.S.F cytology

 

12.                               Final staining exam (specimen is taken from oral

                                    buccal cavity)

 

13.                               REVISION 

 

14.                               FINAL PRACTICAL EXAM 

                                         

 

Assessments:

 

First Mid Term Examination:        15     

Second Mid Term Examination:    15

Laboratory Quizzes:                              5

Mid Term Practical Examination:  5

Final Practical Examination:         20

Final Theoretical Examination:      40

 

References:

 

Chandra Grubb, Diagnostic Cytopathology, Churchill Livingstone, London &New York.