Aspergillus sydowi Metabolites Efficacies against the Mosquito Larval (Culexpipiens and Aedescaspius) Population and Cytotoxicity after Purification with Column Chromatography

Journal Article

مستخلص المنشور

The present study was carried out to evaluate the larvicidal activity and cytotoxicity of Aspergillus sydowi extract. The LC50 calculated of methanol crude extract and fraction 6 was 250.86±4.15 and 56.61±0.89 μg/ml for Aedes caspius and 271.02±7.01 and 103.74±0.99 for Culex pipina respectively after 24 hours of exposure. Larvae of Ae. caspius treated with fraction 6 of A. sydowi methanol extract underwent a lethal disruption of the peritrophic membrane and an extensive cellular microvillar disruption. Expression of Bcl-2 representative of antiapoptotic protein was greatly reduced in control when compared to treated group conversely; Bax the pro-apoptotic protein was higher in treated group. The cytotoxicity of fraction 6 and 7 showed 80.25% and 86.3 % survival at the highest concentration (37μg/ml) tested against Hepg2. On the other hand, Jurkat cells lines showed higher sensitivity to the fraction tested. Fraction 6 showed 45.17% survival where as fraction 7 showed 86.34 % survival at the highest concentration tested. The HPLC chromatogram revealed that fraction 6 obtained is not pure and requires further purification and characterization to examine larvicidal and cytotoxic effect