Acute and Subacute Toxicity of Ethyl Acetate Fraction of Cochliobolus spicifer (Nelson) isolated from Phoenix dactylifera(Linnaeus) on Balb/c Mice

Journal Article

مستخلص المنشور

The aim of this study was to assess the toxicological
effects of the ethyl acetate (ETAC) fraction of
Cochliobolus spicifer (Nelson) isolated from Phoenix
Dactylifera (Linnaeus) using Balb/c mice as an animal
model. An acute toxicity study of the C. spicifer EtoAC
fraction was carried out in male Balb/c mice using a
range of doses of the extract (1,000, 2,000 and 4,000
mg/kg body weight). These doses were injected
intraperitoneally and the mice were observed for
mortality for 24 hrs after exposure. A sub-acute toxicity
investigation was performed using a dose of 125 mg/kg
body weight. These doses were injected
intraperitoneally at 24 hour intervals for 14 days.
Pathological alterations in the kidneys and liver were
inspected histologically.
In the acute toxicity study, the Balb/c mice did not show
mortality within 24 hrs of exposure. In the subacute
toxicity study, blood sugar levels were significantly
reduced (p < 0.05) at a dose of 125 mg/kg body weight.
The microscopic investigation of the kidney
architecture of control and treated mice showed a
normal cellular appearance. The histopathological
study of the liver revealed hepatocytes, infiltration with
massive inflammatory cells, dilation in the central vein
and cytoplasmic vacuolization. The C. spicifer fraction
isolated from P. dactylifera was well tolerated when
intraperitonally injected at a highest dose of 4000
mg/kg body weight but toxic when injected for 14 days
at a dose of 125 mg/kg.