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Farid Shokry Ataya

Associate Professor

Teaching Staff Member

كلية العلوم
2A26 Building 5
المنشورات
ورقة مؤتمر
2018
تم النشر فى:

Saeed HM, Ismaeil M, Embaby A, Ataya FS, Malik A, Shalaby M, E-Banna S, Ali AAM, Bassiouny K. Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase. Protein Exp

In a previous study the full-length open reading frame of the Arabian camel, Camelus dromedarius liver cytosolic glucose-6-phosphate dehydrogenase (G6PD) cDNA was determined using reverse transcription polymerase chain reaction. The C. dromedarius cDNA was found to be 1545 nucleotides (accession number JN098421) that encodes a protein of 515 amino acids residues. In the present study, C. dromedarius recombinant G6PD was heterologously overexpressed in Escherichia coli BL21 (DE3) pLysS and purified by immobilized metal affinity fast protein liquid chromatography (FPLC) in a single step. The purity and molecular weight of the enzyme were analyzed on SDS-PAGE and the purified enzyme showed a single band on the gel with a molecular weight of 63.0 KDa. The specific activity was determined to be 2000 EU/mg protein. The optimum temperature and pH were found to be 60 °C and 7.4, respectively. The isoelectric point (pI) for the purified G6PD was determined to be 6.4. The apparent Km values for the two substrates NADP+ and G6P were found to be 23.2 μM and 66.7 μM, respectively. The far-UV circular dichroism (CD) spectra of G6PD showed that it has two minima at 208 and 222 nm as well as maxima at 193 nm which is characteristic of high content of α-helix. Moreover, the far-UV CD spectra of the G6PD in the presence or absence of NADP+ were nearly identical.

نوع عمل المنشور
Research paper
مزيد من المنشورات
publications

Glutathione S. transferases (GSTs; EC. 2.5.1.18) are a large family of multifunctional enzymes that play crucial roles in the metabolism and inactivation of a broad range of xenobiotic compounds.…

بواسطة Fereniki Perperopoulou, Nirmal Poudel, Anastassios C. Papageorgiou, Farid Ataya and Nikolaos E
2022
تم النشر فى:
MPDI
publications

Background: In the wake of the warning by WHO that the prevalence of dementia may have a rise of 125% in the Middle East by 2050, identification of the genetic risk factors in Arab populations is…

بواسطة Saba Abdi, Amani Ahmed Alghamdi, Nouf Nasser Abduallah AlGhunaim, Reem Muteb Almutairi, Farid Shokry Ataya, Mohammed Ghouse Ahmed Ansari, Syed Danish Hussain, Mohammad Masoud, Abir Alamro,Othman T. Almutairi, Nasser M. Al-Daghri, Taim Abdullah Muayqil
2022
تم النشر فى:
Current Pharmaceutical Biotechnology