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Farid Shokry Ataya

Associate Professor

Teaching Staff Member

كلية العلوم
2A26 Building 5
المنشورات
مقال فى مجلة
2006

20. Katrin Fischer, Angel Llamas, Manuel Tejada-Jimenez, Nils Schrader, Jochen Kuper, Farid S. Ataya, Aurora Galvan, Ralf R. Mendel, Emilio Fernandez, and Guenter Schwarz: (2006): Function and Structure of the Molybdenum Cofactor Carrier Protein from Chla

The molybdenum cofactor (Moco) forms the catalytic site in all eukaryotic molybdenum enzymes and is synthesized by a multistep biosynthetic pathway. The mechanism of transfer, storage, and insertion of Moco into the appropriate apo-enzyme is poorly understood. In Chlamydomonas reinhardtii, a Moco carrier protein (MCP) has been identified and characterized recently. Here we show biochemical evidence that MCP binds Moco as well as the tungstate-substituted form of the cofactor (Wco) with high affinity, whereas molybdopterin, the ultimate cofactor precursor, is not bound. This binding selectivity points to a specific metal-mediated interaction with MCP, which protects Moco and Wco from oxidation with t((1/2)) of 24 and 96 h, respectively. UV-visible spectroscopy showed defined absorption bands at 393, 470, and 570 nm pointing to ene-diothiolate and protein side-chain charge transfer bonds with molybdenum. We have determined the crystal structure of MCP at 1.6 Angstrom resolution using seleno-methionated and native protein. The monomer constitutes a Rossmann fold with two homodimers forming a symmetrical tetramer in solution. Based on conserved surface residues, charge distribution, shape, in silico docking studies, structural comparisons, and identification of an anionbinding site, a prominent surface depression was proposed as a Moco-binding site, which was confirmed by structure-guided mutagenesis coupled to substrate binding studies.

رقم المجلد
281
مجلة/صحيفة
J. Biol. Chemistry
الصفحات
30186–30194
مزيد من المنشورات
publications

Glutathione S. transferases (GSTs; EC. 2.5.1.18) are a large family of multifunctional enzymes that play crucial roles in the metabolism and inactivation of a broad range of xenobiotic compounds.…

بواسطة Fereniki Perperopoulou, Nirmal Poudel, Anastassios C. Papageorgiou, Farid Ataya and Nikolaos E
2022
تم النشر فى:
MPDI
publications

Background: In the wake of the warning by WHO that the prevalence of dementia may have a rise of 125% in the Middle East by 2050, identification of the genetic risk factors in Arab populations is…

بواسطة Saba Abdi, Amani Ahmed Alghamdi, Nouf Nasser Abduallah AlGhunaim, Reem Muteb Almutairi, Farid Shokry Ataya, Mohammed Ghouse Ahmed Ansari, Syed Danish Hussain, Mohammad Masoud, Abir Alamro,Othman T. Almutairi, Nasser M. Al-Daghri, Taim Abdullah Muayqil
2022
تم النشر فى:
Current Pharmaceutical Biotechnology