Apoptotic Potential of Artemsia sieberia Besser (Asteraceae) Fraction against Human Cancer Cell Lines.
Journal Article
, 9. Nael Abutaha*, Ashraf MA Mashaly, Muhammad Farooq and Muhammad A Wadaan. . 2015
Publication Work Type:
Research
Publication Online URL:
Magazine \ Newspaper:
Tropical Journal of Pharmaceutical Research
Issue Number:
10
Volume Number:
14
Pages:
1779-1785
Publication Abstract:
Purpose:
To investigate the antiproliferative and apoptotic activity of crude and dichloromethane fraction of A. sieberi against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 andL929).
Methods:
- sieberi was extracted withmethanol and further purification was carried outusing liquid-liquid extraction with hexane, dichloromethane and ethyl acetate. Each extract was assayed for cytotoxic potential against cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay. The morphology of the HepG2 cell nucleus was investigated by Hoechst 33342, DNA-binding dye. A Tali™ image-based cytometer was used to assess cell viability, death and apoptosis using annexin-v /pi (propidium iodide). A chromatographic fingerprint was constructed using high performance liquid chromatography (HPLC).
- Results:The most effective anticancer activity of the unrefined methanol extract was against HepG2 cell lines (LC50= 161.5 μg/mL). The hexane and ethyl acetate fractions showed no antiproliferative activity. The dichloromethane fraction displayed higher cytotoxic activity (LC50= 61.75 μg/mL) and also repressed the migration of the cells. About 50 % of HepG2 cells were apoptotic when treated for 24 hwith the dichloromethane fraction at the concentration of 120 μg/mL
- Conclusion:A. sieberi possesses apoptotic activity and inhibited the migration of the HepG2 cell lines.