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Hatem A. Abuelizz

Professor

Professor of Medicinal Chemistry

كلية الصيدلة
College of Pharmacy- 2A128
المنشورات
مقال فى مجلة
2015

Distinct substrate specificity and catalytic activity of the pseudoglycosyltransferase VldE

Mahmud, Hatem A Abuelizz, Taifo . 2015

The pseudoglycosyltransferase (PsGT) VldE is a glycosyltransferase-like protein that is similar to trehalose 6-phosphate synthase (OtsA). However, in contrast to OtsA, which catalyzes condensation between UDP-glucose and glucose 6-phosphate, VldE couples two pseudosugars to give a product with an α,α-N-pseudoglycosidic linkage. Despite their unique catalytic activity and important role in the biosynthesis of natural products, little is known about the molecular basis governing their substrate specificity and catalysis. Here, we report comparative biochemical and kinetic studies using recombinant OtsA, VldE, and their chimeric proteins with a variety of sugar and pseudosugar substrates. We found that the chimeric enzymes can produce hybrid pseudo-(amino)disaccharides, and an amino group in the acceptor is necessary to facilitate a coupling reaction with a pseudosugar donor. Furthermore, we found that the N-terminal domains of the enzymes not only play a major role in selecting the acceptors, but also control the type of nucleotidyl diphosphate moiety of the donors.

مجلة/صحيفة
Chemistry & biology
مزيد من المنشورات
publications

The pseudoglycosyltransferase (PsGT) VldE is a glycosyltransferase-like protein that is similar to trehalose 6-phosphate synthase (OtsA). However, in contrast to OtsA, which catalyzes condensation…

بواسطة Hatem A Abuelizz, Taifo Mahmud
2015
publications

The title compound, C18H20N4O2S2, is a new 1,3,4-oxa­diazole and a key pharmacophore of several biologically active agents. It is composed of a meth­yl(thio­phen-2-yl)-1,3,4-oxa­diazole-2(3H…

بواسطة Monirah A Al-Alshaikh, Hatem A Abuelizz, Ali A El-Emam, Mohammed SM Abdelbaky, Santiago Garcia-Granda
2016
publications

C31H36F2N6S, monoclinic, P21/c (no. 14), a = 14.7561(8) Å, b = 24.6766(13) Å, c = 7.7811(4) Å, β = 95.888(2)°, V = 2818.4(3) Å3, 

بواسطة Monirah A Al-Alshaikh, Hazem A Ghabbour, Hatem A Abuelizz, Obaid S Alruqi, Ali A El-Emam
2016