Isolation and characterization of collagen from the skin, scales and fins of Cyrinus carpio and Hypophthalmichthys molitrix

مقال فى مجلة

2014

تم النشر فى: https://www.researchgate.net/publication/271586857_Isolation_and_characterisati…

 

The study was designed to isolate and characterize the collagen from skin, scales and fins of Cyprinus carpio and

Hypophthalmichthys molitrix. For this purpose nine fishes of each species of three weight groups were collected and

categorized as W1(250-500 g), W2(501-750 g) and W3(751- 1000 g). The maximum skin protein content was recorded

as 28.13% and 29.13% in C. carpio and H. molitrix, respectively, from the W3. The scales of the W3displayed a

maximum protein contents as 29.91 and 28.38% for C. carpio and H. molitrix, respectively. The maximum protein

contents (19.19% and 21.37% for C. carpio and H. molitrix, respectively) were observed in the W3group. Protein

characterisation using SDS-PAGE revealed the molecular weights (kDa) of the C. carpio skin, scales, and fins ranged

from 126 kDa to 220 kDa, 78 kDa to 211 kDa, and 72 kDa to 141 kDa, respectively. The size of the collagen proteins of

the H. molitrix skin, scales and fins ranged from 117 kDa to 208 kDa, 81 kDa to 219 kDa, and 72 kDa to 141 kDa,

respectively. Glycine was the most abundant amino acid, whereas, Tryptophan was totally absent in all selected tissues.

Significant differences were observed for amino acids within the weight groups and between the two fish species

• مجلة/صحيفة: Journal of Animal and Plant Sciences