تجاوز إلى المحتوى الرئيسي
User Image

إبراهيم بن عبدالله بن إبراهيم السراء

Professor

عضو هيئة تدريس

كلية الصيدلة
مبنى 23، الدور الأرضي, مكتب رقم AA 90
المنشورات
مقال فى مجلة
2017
تم النشر فى:

An environmentally benign HPLC-UV method for thermodynamic solubility measurement of vitamin D3 in various (Transcutol + water) mixtures

Alsarra, Ibrahim A. . 2017

Dissolution thermodynamics HPLC-UV method Solubility Transcutol® Vitamin D3 Validation

Abstract
In the current research work, an environmentally benign “reversed phase high-performance liquid chromatography (RP-HPLC)” method was developed and validated for thermodynamic solubility measurement of vitamin D3 in various [2-(2-ethoxyethoxy)ethanol (Transcutol®) + water] mixtures. The HPLC analysis of vitamin D3 was achieved using a Nucleodur (150 × 4.6 mm, 5 μm) column. The binary mixture of ethanol: methanol (50:50% v/v) was used as a mobile phase and delivered at a flow rate of 1.0 mL min− 1. The proposed HPLC-UV method was validated well for linearity (R2 = 0.9997), selectivity, accuracy as % recovery (98.60–102.00%), precision (% RSD = 1.07–1.31), robustness and sensitivity. The potential of methodology was demonstrated by its application in thermodynamic solubility determination of vitamin D3 in different “Transcutol + water” mixtures at various temperatures. The “mole fraction solubilities (xe)” of vitamin D3 were measured at temperature “T = 273.2 K to 298.2 K” and pressure “p = 0.1 MPa”. Measured xe values of vitamin D3 were correlated well with “Apelblat, van't Hoff and Yalkowsky” models. The highest xe value of vitamin D3 was obtained in neat Transcutol (4.04 × 10− 1 at T = 298.2 K) followed by lowest one in neat water (1.97 × 10− 7 at T = 273.2 K). “Apparent thermodynamic analysis” of solubility values of vitamin D3 showed an “endothermic and entropy-driven dissolution” of vitamin D3. Overall, these results showed that the proposed HPLC-UV method could be successfully used for thermodynamic solubility determination of vitamin D3 in various “Transcutol + water” mixtures

نوع عمل المنشور
Research Paper
رقم الانشاء
242
مجلة/صحيفة
Journal of Molecular Liquids
الصفحات
from 798 to 806
مزيد من المنشورات