Geographical distribution and molecular detection of Nosema ceranae from indigenous honeybee race of Saudi Arabia

Journal Article

Ansari, Mohammad Javed . 2017

• Publication Work Type: Project
• Publication Online URL: Geographical distribution and molecular detection of Nosema ceranae from indigenous honeybee race of Saudi Arabia
• Tags: Nosema ceranae, Prevalence; Molecular detection, Honeybee, Saudi Arabia
• Magazine \ Newspaper: Saudi Journal of Biological Sciences

Publication Abstract

The aim of the study was to detect the infection level of honey bees with Nosema apis and/or Nosema ceranae using microscopic and molecular analysis from indigenous honeybee race of eight Saudi Arabian geographical regions. A detailed survey was conducted and fifty apiaries were chosen at random from these locations. Infection level were determined both by microscope and Multiplex-PCR and data was analyzed using bioinformatics tools and phylogenetic analysis. Result showed that N. ceranae was the only species infecting indigenous honeybee colonies in Saudi Arabia. As determined by microscope, Nosema spores were found to be in 20.59% of total samples colonies, while 58% of the samples evaluated by PCR were found to be positive for N. ceranae, with the highest prevalence in Al-Bahah, a tropical wet and dry climatic region. Whereas low prevalence was found in the regions with hot arid climate. Honeybees from all eight locations surveyed were positive for N. ceranae. This is the first report about the N. ceranae detection, contamination level and distribution pattern in Saudi Arabia.