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Dr. Javed Ahmad

Assistant Professor

Assistant Professor

كلية العلوم
Room # 2B 121, Second Floor, Bld # 5, Department of Zoology, College of Science
المنشورات
مقال فى مجلة
2013

Zinc ferrite nanoparticles activate IL-1b, NFKB1, CCL21 and NOS2 signaling to induce

Musarrat, Quaiser Saquib · Abdulaziz A Al-Khedhairy · Javed Ahmad ·Maqsood A Siddiqui · Sourabh Dwivedi · Shams T Khan · Javed . 2013

Abstract

The present study has demonstrated the translocation of zinc ferrite nanoparticles (ZnFe2O4-NPs) into the cytoplasm of human amnion epithelial (WISH) cells, and the ensuing cytotoxicity and genetic damage. The results suggested that in situ NPs induced oxidative stress, alterations in cellular membrane and DNA strand breaks. The [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) and neutral red uptake (NRU) cytotoxicity assays indicated 64.48 ± 1.6% and 50.73 ± 2.1% reduction in cell viability with 100 μg/ml of ZnFe2O4-NPs exposure. The treated WISH cells exhibited 1.2-fold higher ROS level with 0.9-fold decline in membrane potential (ΔΨm) and 7.4-fold higher DNA damage after 48 h of ZnFe2O4-NPs treatment. Real-time PCR (qPCR) analysis of p53CASP 3 (caspase-3), and bax genes revealed 5.3, 1.6, and 14.9-fold upregulation, and 0.18-fold down regulation of bcl 2 gene vis-à-vis untreated control. RT2Profiler™ PCR array data elucidated differential up-regulation of mRNA transcripts of IL-1bNFKB1NOS2 and CCL21 genes in the range of 1.5 to 3.7-folds. The flow cytometry based cell cycle analysis suggested the transfer of 15.2 ± 2.1% (p < 0.01) population of ZnFe2O4-NPs (100 μg/ml) treated cells into apoptotic phase through intrinsic pathway. Over all, the data revealed the potential of ZnFe2O4-NPs to induce cellular and genetic toxicity in cells of placental origin. Thus, the significant ROS production, reduction in ΔΨm, DNA damage, and activation of genes linked to inflammation, oxidative stress, proliferation, DNA damage and repair could serve as the predictive toxicity and stress markers for ecotoxicological assessment of ZnFe2O4-NPs induced cellular and genetic damage.

رقم المجلد
273
رقم الانشاء
2
مجلة/صحيفة
Toxicology and Applied Pharmacology
الصفحات
289-297
مزيد من المنشورات
publications

Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…

بواسطة Maqsood A. Siddiqui , Rizwan Wahab , Quaiser Saquib , Javed Ahmad , Nida N. Farshori , Ebtesam S. Al-Sheddi , Mai M. Al-Oqail , Shaza M. Al-Massarani , Abdulaziz A. Al-Khedhairy.
تم النشر فى:
ELZEVIER
publications

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…

2023
تم النشر فى:
ELZEVIER
publications

Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…

بواسطة Javed Ahmad a, Rizwan Wahab, Maqsood A. Siddiqui, Quaiser Saquib, Abdulaziz A. Al-Khedhairy.
2023
تم النشر فى:
ELZEVIER