تجاوز إلى المحتوى الرئيسي
User Image

Dr. Javed Ahmad

Assistant Professor

Assistant Professor

كلية العلوم
Room # 2B 121, Second Floor, Bld # 5, Department of Zoology, College of Science
المنشورات
مقال فى مجلة
2016

Hazards of low dose flame-retardants (BDE-47 and BDE-32): Influence on transcriptome regulation and cell death in human liver cells

Al-Arifi, Quaiser Saquib · Maqsood A. Siddiqui · Javed Ahmed · Abdullah Al-Salim · Sabiha M. Ansari · Mohammad Faisal · Abdulaziz A. Al-Khedhairy · Javed Musarrat · Hend A. AlWathnani · Abdulrahman A. Alatar · Saud A . 2016

Abstract
We have evaluated the in vitro low dose hepatotoxic effects oftwo flame-retardants (BDE-47 and BDE-32) in HepG2 cells. Both congeners declined the viability of cells in MTT and NRU cell viability assays. Higher level of intracellular reactive oxygen species (ROS) and dysfunction of mitochondrial membrane potential (m) were observed in the treated cells. Comet assay data confirmed the DNA damaging potential of both congeners. BDE-47 exposure results in the appearance of subG1 apoptotic peak (30.1%) at 100 nM, whileBDE-32 arrestedthe cells in G2/Mphase.Among the set of 84 genes,BDE-47 inducesdownregulation of majority of mRNA transcripts, whilst BDE-32 showed differential expression of transcripts in HepG2. The ultrastructural analysis revealed mitochondrial swelling and degeneration of cristae in BDE-47 and BDE-32 treated cells. Overall our data demonstrated the hepatotoxic potential of both congeners via alteration of vital cellular pathways. 

نوع عمل المنشور
KSU Research work
رقم المجلد
308
مجلة/صحيفة
Journal of Hazardous Materials
الصفحات
37-49
مزيد من المنشورات
publications

Background: Nanotechnology and material science have developed enormously fast in recent years. Due to their excellent magnetic properties, iron oxide nanoparticles (IONPs) have been broadly…

بواسطة Maqsood A. Siddiqui , Rizwan Wahab , Quaiser Saquib , Javed Ahmad , Nida N. Farshori , Ebtesam S. Al-Sheddi , Mai M. Al-Oqail , Shaza M. Al-Massarani , Abdulaziz A. Al-Khedhairy.
تم النشر فى:
ELZEVIER
publications

Background: Colorectal cancer (CRC) is the third most diagnosed cancer in the world. Artemisia monosperma (family: Asteraceae), a flowering plant species in the genus Artemisia, is widely used in…

2023
تم النشر فى:
ELZEVIER
publications

Vanadium oxide is largely applied as a chemical and industrial product in various field such as in electro- chemical, photochemical and refining industries. Very limited studies are available for…

بواسطة Javed Ahmad a, Rizwan Wahab, Maqsood A. Siddiqui, Quaiser Saquib, Abdulaziz A. Al-Khedhairy.
2023
تم النشر فى:
ELZEVIER