Identification of TRAP and SRAP markers linked with yield components under drought stress in wheat (Triticum aestivum L.)

Journal Article

Barakat1,5, Khaled A. Moustafa1, M. Saleh2, A. A. Al-Doss1, A. A. Elshafei1,3, A. K. Salem1,4, F. H. Al- Qurainy2, M. N. . 2014

• المجلة \ الصحيفة: POJ 2014)
• رقم الإصدار السنوي: 7(4):(
• الصفحات: 253-259

مستخلص المنشور

Abstract

In order to identify TRAP and SRAP markers linked to yield components under drought stress in wheat (

 

Triticum aestivum L.), a segregating F4 population from the cross between drought-sensitive (Yecora Rojo) and drought-tolerant (Pavon 76) genotypes was generated. The parents and 150 F4 families were evaluated phenotypically for drought tolerance using two irrigation treatments [2.5 and 7.5 m3(H2O) m-2(soil)]. The polymorphism among parental genotypes and F4 families were tested using 40 and 98 different TRAP and SRAP primer combinations, respectively. The results revealed that ten of fourteen TRAP and nine of nineteen SRAP markers are linked to the agronomic traits and can be introduced as new markers. The 14 TRAP markers were assigned to chromosomes 2A, 4A, 5A, 1B, 3B, 6B and 2D. The 19 SRAP markers were assigned to chromosomes 2A, 4A, 5A, 7A, 1B, 2B, 3B, 1D and 3D. Results also showed that all of the QTLs had a positive additive effect on agronomic traits, indicating contribution of alleles by the tolerant parent 'Pavon76'. QTLs for the six agronomic traits was associated with above mentioned markers and explained from 5 to 42 % of the phenotypic variation for all the agronomic traits. The genetic distance ranged from 10.1 to 38.1 cM. Therefore, the TRAP and SRAP markers linked to the QTL for the drought tolerance can be further used in breeding for drought tolerance in wheat.

Keywords:

 

agronomic traits, QTL analysis, water stress.