Prevalence, plasmids and antibiotic resistance correlation of enteric bacteria in different drinking water resources in Sohag, Egypt.
Journal Article
AbdelRahim, Khalid . 2015
Publication Work Type:
بحث
Magazine \ Newspaper:
Jundishapur Journal of Microbiology ):
Issue Number:
1
Volume Number:
8
Pages:
e18648. DOI: 10.5812/jjm.18648
Publication Abstract:
Background:
One of the major health causing problems, is contamination of drinking water sources with human pathogenic bacteria. The enteric bacteria such as Shigella, Salmonella, E. coli are the much enteric bacteria causing serious health problems. Occurrence of such these bacteria which may resist antibiotics, increase the seriousness of the problem.
Objective:
Is to examine the prevalence of some enteric bacteria (Shigella, Salmonella, E. coli) in addition to Pseudomonas. The antibiotic susceptibility of these bacteria will also being tested, in addition of suggestion of plasmid(s) roles in supposed resistance. The MRSA genes in non-staphylococci will be clarified.
Material and Methods:
Water samples were collected from different drinking sources (Nile, ground water) and treated tap water. Selective media were used to isolate enteric bacteria and Pseudomonas. These bacteria were identified, counted, and examined for its susceptibility towards 10 antibiotics. The plasmid were screened in these strains, MRSA genes were also examined using PCR.
Results:
Thirty two bacterial strains were isolated from Nile and ground water and identified as Shigella flexneri, Shigella sonnie, Salmonella serovar Newport, Pseudomonas aeruginosa and E. coli strains according to standard methods.
According to antibiotic susceptibility test, 81% of strains were resistant to Cefepim, whereas 93.75% were sensitive to Ciprofloxacin. Correlation analysis between plasmids profiles and antibiotics sensitivities showed that, fifty percent of the total strains were possessing plasmids. These strains showed resistance to 50% of the used antibiotics (as average value), whereas the plasmids free strains (50%) resist 48.7% of the antibiotics. No distinct correlation between plasmids and antibiotic resistance in some strains could be concluded in this study. No MRSA gene was detected among these non-staphylococci strains. No bacteria were isolated from treated tap water.
Conclusion:
Thirty three bacterial strains; 10 strains of E. coli, 10 strains of Shigella flexneri, 3 strains Shigella sonnie, 2 strains of Salmonella serovar Newport, and 7 strains of Pseudomonas aeruginosa, were isolated and identified from Nile water and ground water in Sohag governorate. The prevalence of enteric bacteria in water sources in studying area was considerable. No clear or distinct correlation between plasmids and antibiotic resistance could be concluded. No MRSA gene was detected among these non-staphylococci strains, and no pathogenic bacteria were isolated from treated tap water. The hygiene procedures in the studying area seem to be adequate, despite the failure to maintain the water sources form sewage pollution.