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Dr. Khalid M. Al-Anazi د. خالد بن مشاي العنزي

Professor

Professor of Genetics

كلية العلوم
College of Science – Zoology Department B.O.P.: 57788, Office n. 2B 162
المنشورات
مقال فى مجلة
2016

Assessment of biological activity and UPLC–MS based chromatographic profiling of ethanolic extract of Ochradenus arabicus

2016, Ali, Ali MA, Farah MA, Al-Hemaid FM, Abou-Tarboush FM, Al-Anazi KM, Wabaidur SM, Alothman ZA, Lee J. . . 2016

Ochradenus arabicus Cytotoxicity ROS Oxidative stress UPLC–MS

Natural products from wild and medicinal plants, either in the form of crude extracts or pure compounds provide unlimited opportunities for new drug leads owing to the unmatched availability of chemical diversity. In the present study, the cytotoxic potential of crude ethanolic extract of Ochradenus arabicus was analyzed by MTT cell viability assay in MCF-7 adenocarcinoma breast cancer cells. We further investigated its effect against oxidative stress induced by anticancer drug doxorubicin. In addition, Ultra Performance Liquid Chromatography–Mass Spectrometry (UPLC–MS) based chromatographic profiling of crude extract of O. arabicus was performed. The MTT assay data showed that the extract is moderately toxic to the MCF-7 cells. However, its treatment alone does not induce oxidative stress while doxorubicin increases the level of oxidative stress in MCF-7 cells. Whereas, simultaneous treatment of plant extract and doxorubicin significantly (p < 0.05) decreased the level of intracellular reactive oxygen species (ROS) and lipid peroxidation while an increase in the reduced glutathione and superoxide dismutase activity was observed in time and dose dependent manner. Hence, our finding confirmed cytotoxic and antioxidant potential of crude extract of O. arabicus in MCF-7 cells. However, further investigations on O. arabicus as a potential chemotherapeutic agent are needed. The analysis of bioactive compounds present in the plant extracts involving the applications of common phytochemical screening assays such as chromatographic techniques is discussed.

 

 

 

نوع عمل المنشور
KSU Research Work
مجلة/صحيفة
Saudi Journal of Biological Sciences
الصفحات
229-236
مزيد من المنشورات
publications

Psoriatic inflammation has been shown to be associated with cardiovascular dysfunction and systemic inflammation. Recently, psoriasis has also been linked to hepatic disorders, however…

بواسطة Naif O.Al-HarbiaAhmedNadeemaMohammed M.Al-HarbiaKhairy M.A.ZoheiraMushtaq A.AnsariaAhmed M.El-SherbeenybKhalid M.AlanazicMoureq R.AlotaibiaSheikh F.Ahmada
2017
publications

Objective

This study was designed to characterize the DNA polymorphisms of the melanocortin-1 receptor (MC1R) gene in indigenous Saudi Arabian sheep breeds exhibiting different…

بواسطة hmed H. Mahmoud , Ashraf M. Mashaly , Ahmed M. Rady , Khalid M. Al-Anazi , and Amgad A. Saleh.
2017
publications

ABSTRACT. The bioactive compounds proceraside A, frugoside and calotropin, which were extracted from the root bark of Calotropis procera (Aiton) W.T. Aiton (family Asclepiadaceae), were recently…

بواسطة Gurung, A.B. Gurung , M.A. Ali , A. Bhattacharjee , M. AbulFarah , F. Al-Hemaid , F.M. Abou-Tarboush , K.M. Al-Anazi , F.S.M. Al-Anazi and J. Lee, . 2016
2016