Effect of Inorganic Mercury on Drug Metabolizing Enzymes of Promethazine-Pretreated and Phenobarbitone–Pretreated Male Rabbit
Shakoori, Farah Anjum,1 Farah R. Shakoori,1 K.A. Al-Ghanim2 and Abdul Rauf . 2016
Effects of inorganic mercury have been studied on the drug metabolizing enzymes (DME) of
phenobarbitone- (PB-) and promethazine- (PM-) pretreated male New Zealand white rabbit,
Oryctolagus cunciculus. After PB treatment, the activities of cytochrome p-450 (CP), aniline
hydroxylase (AH), acetanilide hydroxylase (AAH), benzphatamine demethylase (BD), aminopyrine
demethylase (APD) and N.N dimethyl aniline demethylase (DAD) increased 214, 266, 207, 108, 81
and 83%, respectively. Cytochrome-c-reductase (CCR) also increased 109%. However, the activities
of cytochrome b5 (CB) and N.N dimethyl aniline N-oxide (DAO) decreased 76% and 38%,
respectively. PM administration had no effect on any of the DMEs except for AH, AAH and BD,
which showed 60, 5 and 16% increase, respectively. HgCl2 administered at a dose of 1mg/kg body
wt per day for 5 days did not affect the CP, CB, APD and CCR. The activities of AH and AAH
increased 32 and 33%, respectively, whereas those of BD, DAD, and DAO decreased 35%, 30% and
32%, respectively. When administered to PB-pretreated animals CP, AH, AAH, BD, APD, DAD,
CCR, and DAO were inhibited 57, 58, 42, 31, 56, 55, 76 and 59%, respectively. Activity of CB
increased 351%. HgCl2 had no effect on CP and CCR when administered to PM-pretreated animals.
AH, AAH and CB increased 17, 135 and 30%, respectively. BD, APD and DAD were reduced 47,
10 and 19, respectively. Formation of DAO was also inhibited 52%. To conclude, mercury
drastically interferes with the DME. The metabolism of PB is inhibited, whereas that of PM is
variably affected – hydroxylases are up-regulated and demethylases are down-regulated. CB is
however, induced 351% after mercury treatment.
This current work revealed a single-step fabrication of tungsten oxide nanoflakes (WO3
NFs) with the help of Terminalia arjuna bark extract. Bioactive phytoconstituents of T. arjuna bark…
In the present work, the residual biomass of the green seaweed Ulva lactuca was chosen as feedstock to undergo separate
hydrolysis and fermentation process to produce bioethanol. The…
Waterbirds may be a good indicator of harmful metal levels in aquatic environments. Waterbirds’
organs and tissues were tested for the presence of pollutants, such as metals. However,
…