Effect of an Organophosphate Insecticide Diazinon on the Activity of Acetylcholinesterase and Lipid Peroxidation of a Common Carp, Cyprinus Carpio L.
Al-Ghanim*, Khalid Abdullah . 2014
Diazinon is a widely used organophosphorus pesticide in agriculture and environmental health ,
hence its adverse effects on non target animals, especially on fish is to be determined. The present research is
conducted to study the influence of diazinon [0, 0-diethyl 0-(2-isopropyl-6-methylpyrimidin-4yl) phosphorothioate]
on acetylcholinestrase (AChE) and lipidperoxidation (LPO) on freshwater common carp (Cyprinus carpio L.). Adult
fish (mean body weight 42.6±3.86 g and 19.1±2.66 cm mean length ) were divided into five groups with 3 replicates
and subjected to 5 concentrations (100, 200, 300, 400 and 500 μg L-1) of diazinon for 30 days and compared with
control (untreated) fish. Fishes were acclimatized to laboratory conditions for 7 days and then Physio-chemical
characteristic of water was determined before the treatment. Induction of oxidative stress in various tissues was
evidence of increased lipid peroxidation levels seems to be associated with the concentration of diazinon.
Acetylcholinesterase activity responded positively in a concentration dependent pattern. It was concluded that LPO
and AChE could be a valuable biomarker as an indicator of water pollution on aquatic ecosystems.
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