Neospora caninum jumps species barrier

Conference Paper

• اسم المؤتمر: Society for General Microbiology Autumn Conference 2012
• عنوان المؤتمر: Warwick, UK
• تاريخ المؤتمر: الاثنين, أيلول (سبتمبر) 3, 2012

مستخلص المنشور

n a previous study we showed for the first time that the invertebrate locust is permissible to infection with the apicomplexan protozoan parasite Neospora caninum. A viable strain of N. caninum was recovered from experimentally infected locusts and maintained in vitro via serial passages in cultured endothelial cells for up to 4 months before being stored in liquid nitrogen.  Herein, we compared the genetic difference and chemical profile of N. caninum strain isolated from brain of infected locust to original strain normally maintained in tissue culture.  Locust-derived strain produced more productive infection compared to the original strain.  However, PCR-sequencing analysis based on N. caninum species-specific primer pair Np21plus–Np6plus which anneals into the Nc5 region revealed that both parasite strains are identical.  Also, we used the micro-Raman spectroscopy technique and Principal Component Analysis to identify the chemical structural differences between the two strains and minor, non-significant differences were found between the 2 strains.  These results indicate that the current definition of the species barrier, which has been based on host specificity, needs to be reassessed.