السيرة الذاتية

التعريف الشخصي: 
As a graduate student under the supervision of Dr. Humaira Gowher at Purdue University’s Department of Biochemistry, I had the privilege of collaborating with talented members of Gowher lab and contributed many publications. My research involved the use of embryonic stem cells and embryonal carcinoma cell differentiation as model system to study how enhancers fail to be silenced in cancer stem cells. Further, in our manuscript submitted to Molecular Cell, we determined a mechanism by which these enhancers acquire a primed state prone to activation. These primed enhancers are what render these cells plastic under the many environmental conditions. In order to decipher how DNA methyltransferases are recruited and targeted to specific sites and how this mechanism is disrupted in cancer, I refined a DNA methyltransferase capture method. By the use of a suicide inhibitor, we demonstrated that we could capture DNA methyltransferases as they exert their catalytic activity onto their DNA substrate. This novel method can be used to investigate dynamic changes of DNA methylation deposited by specific DNA methyltransferases during cellular differentiation. Additionally, I am a co-author on a 2018 publication in J. Biol. Chem., where we illustrated how the transcription factor Vezf1 is critical for endothelial lineage determination via regulating the expression of stem cell factor, Cbp/p300-interacting transactivator 2 (Cited2). We showed that Vezf1 depleted cells have higher expression of Cited2. Given Vezf1 reported role as an insulator protein, our data highlights a mechanism by which Vezf1 occupancy at Cited2 promoter prevents aberrant promoter activation by blocking inappropriate interactions of promoters with nearby enhancers. Since becoming part of Dr. Gowher’s laboratory in 2015, I have been able to co-author in two published manuscripts and one, which I am the first co-author. I also attended many national conferences such as the Midwest Chromatin and Epigenetics Meeting and American Society for Biochemistry and Molecular Biology Meeting to name a few. I also mentored seven ambitious and talented undergraduate students as well as several graduate students during their rotations periods in the lab. My experiences in research and training acquired in US equipped me to perform my role as an academic in Saudi Arabia.
ملف مرفق: 
المرفقالحجم
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