Evaluating the Antibacterial Activity of Fenugreek (Trigonella foenum-graecum) Seed Extract against A Selection of Different Pathogenic Bacteria

Journal Article
, Mona S. alWhibi . 2014
Publication Work Type: 
بحث منشور
Tags: 
http://fac.ksu.edu.sa/sites/default/files/mona_s_alwhibi-1.pdf
Magazine \ Newspaper: 
JOURNAL OF PURE & APPLIED MICROBIOLOGY
Issue Number: 
Vol. 8
Volume Number: 
Nov. 2014
Pages: 
Vol. 8(Spl. Edn. 2), p. 817-821
Publication Abstract: 

In the present study, Fenugreek plants obtained from two different cultivars, one from Saudi Arabia and another from Yemen, were screened for phytochemical active constituents and investigated for antimicrobial properties against a selection of grampositive and gram-negative pathogenic bacteria. Five different solvent seed extracts from each cultivar were tested. The results of the study demonstrated that the chloroform and methanolic extracts possessed significant antibacterial activity against Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC25923, Salmonella typhi ATCC14027 and Klebsiella pneumonia ATCC700603. The antimicrobial activity of the extracts was investigated using the agar well diffusion method. The results of the antimicrobial analysis identified Shigella sonnei as the most sensitive pathogen to the crude extracts of Fenugreek seeds obtained from Saudi Arabia and with the largest zones of inhibition.