Quantitative proteomic profiling of immune responses to Ichthyophthirius multifiliis in common carp skin mucus

Journal Article
Publication Work Type: 
original article
Magazine \ Newspaper: 
Fish and Shellfish Immunology
Issue Number: 
1
Volume Number: 
84
Pages: 
834-845
Publication Abstract: 

Ichthyophthirius multifiliis, a ciliated protozoan parasite, causes ichthyophthiriasis and leads to considerable
economic losses to the aquaculture industry. Understanding the fish immune response and host-parasite interactions
could support developing novel strategies for better disease management and control. Fish skin mucus is
the first line of defence against infections through the epidermis. Yet, the common carp, Cyprinus carpio, proteinbased
defence strategies against infection with I. multifiliis at this barrier remain elusive. The skin mucus proteome
of common carp was investigated at 1 day and 9 days post-exposure with I. multifiliis. Using nano-LC ESI
MS/MS and statistical analysis, the abundance of 19 immune related and signal transduction proteins was found
to be differentially regulated in skin mucus of common carp in response to I. multifiliis. The analysis revealed
increased abundance values of epithelial chloride channel protein, galactose-specific lectin nattection, high
choriolytic enzyme 1 (nephrosin), lysozyme C, granulin and protein-glutamine gamma-glutamyltransferase 2 in
I. multifiliis-exposed carp skin mucus. Multiple lectins and a diverse array of distinct serpins with protease inhibitor
activity were identified likely implicated in lectin pathway activation and regulation of proteolysis,
indicating that these proteins contribute to the carp innate immune system and the protective properties of skin
mucus. The results obtained from this proteomic analysis enables a better understanding of fish host response to
parasitic infection and gives insights into the key role skin mucus plays in protecting fish against deleterious
effects of I. multifiliis.

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