Cloning, sequencing and expressing of the carotenoid biosynthesis genes of ß-carotene from epiphytic bacteria Erwinia uredovora in non carotenogenic bacteria E. coli.

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Abstract: Four genes which encode the enzymes of ß-carotene in the epiphytic bacteria Erwinia uredovora have
been designated as crtE, crtB, crtI and crtY. These genes were cloned in Eschershia coli BL21 and located on a
4467-bp fragment whose nuclotide sequence was determined. E. coli does not naturally synthesize carotenoids but,
by using the carotenogenic genes recombinant strains accumulated ß-carotene where the four genes were expressed
as follows: farnesyl diphosphate (FPP) crtE geranylgeranyl diphosphate (GGPP) crtB phytoene crtI lycopene crtY
ß-carotene. E. coli BL21 acquired the yellow pigments due to the prescence of ß-carotene after transformed by
pGEX-5X-3-crtEBIY.
[Hany M. Yehia, Alaeldein M. Abudabos, Manal F. Elkhadragy, Ebtesam M. Al- Olayan, Abd-El-Rahman M.
Khalaf- Allah and Nagwa M. El-Shimi. Cloning, sequencing and expressing of the carotenoid biosynthesis genes
of ß-carotene from epiphytic bacteria Erwinia uredovora in non carotenogenic bacteria E. coli. Life Sci J 2013;
10(3): 1904-1917] (ISSN: 1097-8135). http://www.lifesciencesite.com. 283
Key Words: Erwinia uredovora, carotenoid genes, Eschershia coli BL21.

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