Mitigation of Ni toxicity by exogenous application of SA and NO in wheat
Journal Article
Basalah, Manzer H. Siddiqui, M.H. Al-Whaibi, H.M. Ali, A.M. Sakran and M.O. . 2013
Publication Online URL:
Issue Number:
11
Volume Number:
7
Pages:
1780 to1788
Publication Abstract:
Nitric oxide and salicylic acid are signaling and ubiquitous bioactive molecules that regulate cellular mechanism in plants under abiotic stress. Therefore, the present experiment was conducted to study the interactive effect of NO and/or SA in tolerance of wheat (Triticum aestivum L. cv. Samma) to nickel stress. The treatments were given as follows: (1) Ni0 mM + NO0 mM+ SA0 mM (control), (2) Ni1 mM + NO0 mM+ SA0 mM, (3) Ni0 mM + NO0.5 mM+ SA0 mM, (4) Ni0 mM + NO0 mM+ SA0.5mM, (5) Ni1 mM + NO0.5 mM+ SA0 mM, (6) Ni1 mM + NO0 mM+ SA0.5 mM, (7) Ni0 mM + NO0.5 mM+ SA0.5 mM, (8) Ni1 mM + NO0.5 mM+ SA0.5 mM. The results showed Ni-fed plants exhibited moderate reduction in growth characteristics (PH, FW, DW and LA). Also, the application of Ni inhibited CA activity, content of essential elements (N, P, K) and Chl. Plants showed higher accumulation of Ni and MDA under Ni stress. Under Ni stress, the combined application of NO and SA induced the activities of enzymes (CA, SOD, POD, CAT) and accumulation of osmoprotectant proline (Pro). Also, combined application of SA and NO improved the photosynthetic pigments (Chl a and Chl b) and maintained nutrients homeostasis in plants under Ni-stress. Application of NO and SA together was more effective in suppression of deleterious effect of Ni stress by reducing MDA formation in plants. The present study reveals that application of NO and SA together improved plant growth and development by improving activity of antioxidant enzymes, CA and balance supply of nutrients.