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محمد بن عبدالرحمن السنيدي

Associate Professor

رئيس قسم الصيدلانيات

كلية الصيدلة
قسم الصيدلانيات, كلية الصيدلة, مبنى رقم 23 ,الدور الأرضي , مكتب أأ 101
المنشورات
مقال فى مجلة
2011

An empirical phase diagram approach to investigate conformational stability of “second-generation” functional mutants of acidic fibroblast growth factor (FGF-1)

السنيدي, محمد . 2011

Acidic fibroblast growth factor (FGF-1) is an angiogenic protein which requires binding to a polyanion such as heparin for its mitogenic activity and physicochemical stability. To evaluate the extent to which this heparin dependence on solution stability could be reduced or eliminated, the structural integrity and conformational stability of ten selected FGF-1 mutants were examined as a function of solution pH and temperature by a series of spectroscopic methods including circular dichroism, intrinsic and extrinsic fluorescence spectroscopy and static light scattering. The biophysical data were summarized in the form of colored empirical phase diagrams (EPDs). FGF-1 mutants were identified with stability profiles in the absence of heparin comparable to that of wild type FGF-1 in the presence of heparin while still retaining their biological activity. In addition, a revised version of the EPD methodology was found to provide an information rich, high throughput approach to compare the effects of mutations on the overall conformational stability of proteins in terms of their response to environmental stresses such as pH and temperature.

نوع عمل المنشور
بحث علمي محكم
رقم المجلد
21
رقم الانشاء
3
مجلة/صحيفة
Protein Science
الصفحات
418-432
مزيد من المنشورات
publications

The structural integrity and conformational stability of various IgG1-Fc proteins produced from the yeast Pichia pastoris with different glycosylation site occupancy (di-, mono-, and…

2014
publications

In this review, some of the challenges and opportunities encountered during protein comparability assessments are summarized with an emphasis on developing new analytical approaches to better…

بواسطة Mohammad A. Alsenaidy, Nishant K. Jain, Jae H. Kim, C. Russell Middaugh and David B. Volkin
2014
publications

Acidic fibroblast growth factor (FGF-1) is an angiogenic protein which requires binding to a polyanion such as heparin for its mitogenic activity and physicochemical stability.

بواسطة محمد السنيدي
2011