PROTEOLYTIC ACTIVITY OF RECENT FORAMINIFERAL FUNGI ISOLATED FROM DIFFERENT COASTAL LAGOONS IN RED SEA COAST, EGYPT

Journal Article
Saleem, Mohamed Youssef and Abdel-Rahman . 2017
Publication Work Type: 
article
Magazine \ Newspaper: 
WulFenia
Issue Number: 
9
Volume Number: 
24
Pages: 
258-270
Publication Abstract: 

Twenty nine fungal species were isolated from 30 samples of picked recent foraminferal tests belong to three different coastal lagoons (Abu-Shaar, Umm al-Huwaytāt and Marsa Shūni lagoons) in red sea coast, Egypt. Aspergillus, Emericella, Paecilomyces, Penicillium and Trichoderma were the most prevalent genera on both dextrose Czapeks and potato dextrose
agar media at 28°C. From the above genera, the most dominant species were Aspergillus flavus, A. niger, A. terreus, Emericella nidulans, Paecilomyces variotii, Penicillium chrysogenum and Trichoderma harzianum. Screening of fungi for their abilities to produce protease enzyme indicated that, twelve species represented 41.38% of total fungi exhibited
high and moderate protease activity each. However, five species (17.24%) were low producers. Aspergillus flavus, Fusarium oxysporum and Penicillium chrysogenum were the most active species for protease production. These fungi exhibited maximum protease activity after 6 days of incubation at 32°C with initial pH 6. The crude enzyme of A. flavus
culture extract was concentrated by ammonium sulphate and dialysis. The partially purified enzyme produced 4.8 fold with recovery of 4 and the molecular weight estimated by SDSPAGE was about 48 kDa.
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