تجاوز إلى المحتوى الرئيسي
User Image

نائل محمود أبوطه

Associate Professor

عضو هيئة تدريس

كلية العلوم
كلية العلوم-كرسي أبحاث المنتجات الحيوية رقم المعمل AB85
المنشورات
مقال فى مجلة
2015

In vitro antiproliferative activity of partially purified Withania somnifera fruit extract on different cancer cell lines

Purpose: Cancer is a major health problem worldwide. There is a continuous need to search for safer and more effective alternatives to overcome the side effects and resistance of the chemotherapeutic agents. Therefore, in this study we investigated the antiproliferative activity and the apoptotic potential of Withania somnifera (W. somnifera).
Methods: W. somnifera was extracted with methanol and then solvent partitioned by sequential extractions with hexane, dichloromethane and ethyl acetate. Each extract was assayed for antiproliferative activity against different cancer cell lines using MTT assay. The nuclear morphology of HepG2 cells was investigated by DNA-binding fluorescent dye (Hoechst 33342 stain). The percentage of viability, death and apoptosis were evaluated by the TaliTM Image-based cytometer using annexin-V/PI (propidium iodide). A chromatographic fingerprint was constructed using high performance liquid chromatography (HPLC).
Results: The most potent anticancer activity of the crude extract was against HepG2 cell line (LC50=164.7μg/ml). Dichloromethane fraction showed remarkable changes in the chromatin structure i.e., fragmentation, uniform condensation. Of the HepG2 cells 43.6% were apoptotic when treated with dichloromethane fraction for 24 hrs at 95μg/ml concentration. HPLC showed the presence of a major peak at 11.85 min.
Conclusion: W. somnifera may have the potential to serve as a template for future anticancer drug development. However, further investigation is required to identify the active compound/s.

رقم الانشاء
20(2)
مجلة/صحيفة
JBUON
الصفحات
625-630
مزيد من المنشورات
publications

Cancer is reported to be the leading cause of death and illness worldwide. This research aims to evaluate the phytochemicals,

بواسطة Ahmed I. Alsayadi1 · Nael Abutaha1 · Bader O. Almutairi1 · Fahd A. Al‑Mekhlaf1 · Mohamed A. Wadaan1
2022
تم النشر فى:
Environmental Science and Pollution Research
publications

The essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty…

بواسطة Ramzi A. Mothana, Fahd A. Nasr, Jamal M. Khaled, Omar M. Noman, Nael Abutaha, Adnan J. Al-Rehaily, Omar M. Almarfadi and Mine Kurkcuoglu
تم النشر فى:
open chemistry
publications

Plants of the Lavandula genus have been widely used in folk and traditional medicine. The aim of this study was to evaluate the qualitative and quantitative chemical composition of the essential…

بواسطة Mohamed HabibOueslatiabNaelAbutahacFarajAl-GhamdidImededdineArbi NehdieFahd A.NasrcLamjedMansourcMohammedAL-ZharanifAbdel HalimHarrath
تم النشر فى:
Journal of King Saud University – Science