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نائل محمود أبوطه

Associate Professor

عضو هيئة تدريس

كلية العلوم
كلية العلوم-كرسي أبحاث المنتجات الحيوية رقم المعمل AB85
المنشورات
مقال فى مجلة
2015

Apoptotic Potential of Artemsia Sieberia Dichloromethane Fraction against Human Cancer Cell Lines. .

Purpose: To investigate the anti-proliferative and apoptotic activity of crude and dichloromethane fraction of A. sieberi against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 and L929).
Methods: A. sieberi was extracted with methanol and further purification was carried out using liquidliquid extraction with hexane, dichloromethane and ethyl acetate. Each extract was assayed for cytotoxic potential against cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay. The morphology of the HepG2 cell nucleus was investigated by Hoechst 33342, DNA-binding dye. A Tali™ image-based cytometer was used to assess cell viability, death and apoptosis using annexin-v /pi (propidium iodide). A chromatographic fingerprint was constructed using high performance liquid chromatography (HPLC).
Results: The most effective anticancer activity of the unrefined methanol extract was against HepG2 cell lines (LC50 = 161.5 μg/mL). The hexane and ethyl acetate fractions showed no antiproliferative activity. The dichloromethane fraction displayed higher cytotoxic activity (LC50 = 61.75 μg/mL) and also repressed the migration of the cells. About 50 % of HepG2 cells were apoptotic when treated for 24 h with the dichloromethane fraction at the concentration of 120 μg/mL
Conclusion: A. sieberi possesses apoptotic activity and inhibited the migration of the HepG2 cell lines.

اسم الناشر
Tropical Journal of Pharmaceutical Research
مزيد من المنشورات
publications

Cancer is reported to be the leading cause of death and illness worldwide. This research aims to evaluate the phytochemicals,

بواسطة Ahmed I. Alsayadi1 · Nael Abutaha1 · Bader O. Almutairi1 · Fahd A. Al‑Mekhlaf1 · Mohamed A. Wadaan1
2022
تم النشر فى:
Environmental Science and Pollution Research
publications

The essential oil of Ducrosia ismaelis Asch. (Apiaceae) that grows wild in Saudi Arabia was investigated utilizing gas chromatography (GC), and gas chromatography-mass spectrometry. Fifty…

بواسطة Ramzi A. Mothana, Fahd A. Nasr, Jamal M. Khaled, Omar M. Noman, Nael Abutaha, Adnan J. Al-Rehaily, Omar M. Almarfadi and Mine Kurkcuoglu
تم النشر فى:
open chemistry
publications

Plants of the Lavandula genus have been widely used in folk and traditional medicine. The aim of this study was to evaluate the qualitative and quantitative chemical composition of the essential…

بواسطة Mohamed HabibOueslatiabNaelAbutahacFarajAl-GhamdidImededdineArbi NehdieFahd A.NasrcLamjedMansourcMohammedAL-ZharanifAbdel HalimHarrath
تم النشر فى:
Journal of King Saud University – Science